IMPC phenotypes (gene matching)
B6.129-Il2rbtm1Mak/Cnbc
| Status | Available to order |
| EMMA ID | EM:06371 |
| International strain name | B6.129-Il2rbtm1Mak/Cnbc |
| Alternative name | IL - 2 Rb |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Il2rbtm1Mak |
| Gene/Transgene symbol | Il2rb |
Information from provider
| Provider | Tak W Mak |
| Provider affiliation | Mak Lab, Campbell Family Breast Cancer reseach Institute |
| Genetic information | Mice defective in IL-2Rb expression were generated by insertion of neomycin resistance cassette into Il2rb gene at exon 6 which encodes a region of the extracellular domain proximal to the transmembrane region. |
| Phenotypic information | Homozygous mice are smaller, have abnormal hair, slow movement and poorly developed external genitals. Death occurs at 12 weeks of age. T cells are spontaneously activated causing exhaustive differentiation of B cells into plasma cells and high serum concentrations of IgG1 and IgE, as well as autoantibodies that cause hemolytic anemia. |
| Breeding history | 10 backcrosses to C57BL/6. |
| References |
|
| Homozygous fertile | no |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | yes |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | heterozygous C57BL/6J, wild-type C57BL/6J |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
- enlarged spleen / MGI
- enlarged lymph nodes / MGI
- abnormal T cell activation / MGI
- abnormal spleen periarteriolar lymphoid sheath morphology / MGI
- abnormal lymphocyte morphology / MGI
- increased anti-double stranded DNA antibody level / MGI
- increased susceptibility to autoimmune hemolytic anemia / MGI
- decreased regulatory T cell number / MGI
- decreased T cell proliferation / MGI
- abnormal response to transplant / MGI
- liver inflammation / MGI
- premature death / MGI
- abnormal immune system organ morphology / MGI
- abnormal T cell clonal deletion / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- abnormal marginal zone B cell morphology / MGI
- increased neutrophil cell number / MGI
- increased granulocyte number / MGI
- abnormal sex gland morphology / MGI
- weight loss / MGI
- abnormal locomotor behavior / MGI
- thymus hypoplasia / MGI
- increased activated T cell number / MGI
- abnormal hair texture / MGI
- increased IgE level / MGI
- increased erythroid progenitor cell number / MGI
- increased anti-nuclear antigen antibody level / MGI
- decreased B cell number / MGI
- decreased double-positive T cell number / MGI
- abnormal common myeloid progenitor cell morphology / MGI
- decreased memory T cell number / MGI
- increased CD4-positive, alpha beta T cell number / MGI
- increased CD8-positive, alpha-beta T cell number / MGI
- increased plasma cell number / MGI
- abnormal B cell activation / MGI
- abnormal mononuclear cell morphology / MGI
- increased IgG1 level / MGI
MGI phenotypes (gene matching)
- increased neutrophil cell number / MGI
- increased granulocyte number / MGI
- abnormal sex gland morphology / MGI
- enlarged spleen / MGI
- enlarged lymph nodes / MGI
- weight loss / MGI
- abnormal locomotor behavior / MGI
- thymus hypoplasia / MGI
- abnormal T cell activation / MGI
- increased activated T cell number / MGI
- liver inflammation / MGI
- abnormal hair texture / MGI
- premature death / MGI
- abnormal spleen periarteriolar lymphoid sheath morphology / MGI
- increased IgE level / MGI
- abnormal lymphocyte morphology / MGI
- abnormal immune system organ morphology / MGI
- increased erythroid progenitor cell number / MGI
- increased anti-double stranded DNA antibody level / MGI
- increased anti-nuclear antigen antibody level / MGI
- increased susceptibility to autoimmune hemolytic anemia / MGI
- decreased regulatory T cell number / MGI
- abnormal T cell clonal deletion / MGI
- decreased B cell number / MGI
- decreased double-positive T cell number / MGI
- decreased T cell proliferation / MGI
- abnormal response to transplant / MGI
- abnormal common myeloid progenitor cell morphology / MGI
- decreased memory T cell number / MGI
- increased CD4-positive, alpha beta T cell number / MGI
- increased CD8-positive, alpha-beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- increased plasma cell number / MGI
- abnormal marginal zone B cell morphology / MGI
- abnormal B cell activation / MGI
- abnormal mononuclear cell morphology / MGI
- increased IgG1 level / MGI
Literature references
- Deregulated T cell activation and autoimmunity in mice lacking interleukin-2 receptor beta.;Suzuki H, Kündig T M, Furlonger C, Wakeham A, Timms E, Matsuyama T, Schmits R, Simard J J, Ohashi P S, Griesser H, ;1995;Science (New York, N.Y.);268;1472-6; 7770771
- Abnormal development of intestinal intraepithelial lymphocytes and peripheral natural killer cells in mice lacking the IL-2 receptor beta chain.;Suzuki H, Duncan G S, Takimoto H, Mak T W, ;1997;The Journal of experimental medicine;185;499-505; 9053450
- Normal regulatory alpha/beta T cells effectively eliminate abnormally activated T cells lacking the interleukin 2 receptor beta in vivo.;Suzuki H, Zhou Y W, Kato M, Mak T W, Nakashima I, ;1999;The Journal of experimental medicine;190;1561-72; 10587347