STOCK Rag2tm1Fwa Cd40tm1Kik Tg(TcraH-Y,TcrbH-Y)71Vbo/Orl

Status

Available to order

EMMA IDEM:07463
International strain nameSTOCK Rag2tm1Fwa Cd40tm1Kik Tg(TcraH-Y,TcrbH-Y)71Vbo/Orl
Alternative nameRag2-/- CD40-/- HY+/+Tg
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCd40tm1Kik, Tg(TcraH-Y, TcrbH-Y)71Vbo
Gene/Transgene symbolCd40, Tg(TcraH-Y,TcrbH-Y)71Vbo, Rag2

Information from provider

ProviderBenedita Rocha
Provider affiliationINSERM U 1020, INSERM
Additional ownerFrederick W. Alt, Hitoshi Kikutani, Harald von Boehmer
Genetic informationMutant mice bearing a transgenic alpha-beta TCR specific for the HY male antigen restricted to major histocompatibility complex (https://www.informatics.jax.org/allele/MGI:3044562), and deficient in the Cd40 gene and the Rag2 recombinase gene (TgRAG2) were used to obtain monoclonal populations of CD8+ T cells.
Phenotypic informationMice will have a single population of lymphocytes, those expressing the TCR transgene.
Breeding historyCurrently bred as homozygotes for the Rag2 and Cd40 knock-out mutations and the TCR transgene.
References
  • A role for CD40 expression on CD8+ T cells in the generation of CD8+ T cell memory.;Bourgeois Christine, Rocha Benedita, Tanchot Corinne, ;2002;Science (New York, N.Y.);297;2060-3; 12242444
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredyes
Immunocompromisedyes

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France
Breeding at archiving centreMales are homozygous knock-out for Rag2 and Cd40, transgenic for the Tg(TcraH-Y,TcrbH-Y)71Vbo (HY) transgene. Females are homozygous knock-out for Rag2 and Cd40, heterozygous or wild-type for the Tg(TcraH-Y,TcrbH-Y)71Vbo (HY) transgene.
Stage of embryos2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • decreased bone mineral density / MGI
  • abnormal dendritic cell physiology / MGI
  • increased susceptibility to bacterial infection / MGI
  • hematopoietic system phenotype / MGI
  • decreased cerebral infarction size / MGI
  • decreased platelet aggregation / MGI
  • decreased trabecular bone mass / MGI
  • decreased compact bone mass / MGI
  • decreased B cell number / MGI
  • immune system phenotype / MGI
  • absent germinal center B cells / MGI
  • decreased neutrophil cell number / MGI
  • abnormal humoral immune response / MGI
  • decreased IgA level / MGI
  • decreased immunoglobulin level / MGI
  • decreased IgE level / MGI
  • decreased susceptibility to type II hypersensitivity reaction / MGI
  • absent spleen germinal center / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • decreased IgG2b level / MGI
  • decreased IgG3 level / MGI
  • increased granulocyte number / MGI
  • abnormal spleen morphology / MGI
  • spleen hypoplasia / MGI
  • decreased body size / MGI
  • arrested B cell differentiation / MGI
  • decreased IgM level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • increased susceptibility to infection / MGI
  • abnormal effector T cell morphology / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • decreased double-positive T cell number / MGI
  • increased macrophage cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • absent mature B cells / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • decreased thymocyte number / MGI
  • lung inflammation / MGI
  • abnormal macrophage physiology / MGI
  • colitis / MGI
  • abnormal T-helper 1 physiology / MGI
  • abnormal response to transplant / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal B cell morphology / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal stomach mucosa morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal B cell number / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
MGI phenotypes (gene matching)
  • decreased bone mineral density / MGI
  • decreased neutrophil cell number / MGI
  • abnormal humoral immune response / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • abnormal dendritic cell physiology / MGI
  • increased susceptibility to bacterial infection / MGI
  • decreased immunoglobulin level / MGI
  • abnormal immunoglobulin level / MGI
  • decreased IgE level / MGI
  • increased IgM level / MGI
  • decreased B cell number / MGI
  • immune system phenotype / MGI
  • hematopoietic system phenotype / MGI
  • decreased susceptibility to type II hypersensitivity reaction / MGI
  • abnormal response to transplant / MGI
  • decreased cerebral infarction size / MGI
  • absent germinal center B cells / MGI
  • absent spleen germinal center / MGI
  • decreased spleen germinal center number / MGI
  • decreased spleen germinal center size / MGI
  • decreased IgG1 level / MGI
  • decreased IgG2a level / MGI
  • decreased IgG2b level / MGI
  • decreased IgG3 level / MGI
  • increased IgG3 level / MGI
  • abnormal lymph node germinal center morphology / MGI
  • decreased platelet aggregation / MGI
  • decreased trabecular bone mass / MGI
  • decreased compact bone mass / MGI
  • impaired humoral immune response / MGI
  • decreased monocyte cell number / MGI
  • increased granulocyte number / MGI
  • decreased bone marrow cell number / MGI
  • alopecia / MGI
  • abnormal digestive system morphology / MGI
  • abnormal intestinal epithelium morphology / MGI
  • abnormal intestinal mucosa morphology / MGI
  • abnormal spleen morphology / MGI
  • small spleen / MGI
  • spleen hypoplasia / MGI
  • abnormal Peyer's patch morphology / MGI
  • abnormal thymus morphology / MGI
  • decreased thymocyte number / MGI
  • abnormal immune system cell morphology / MGI
  • decreased body size / MGI
  • abnormal humoral immune response / MGI
  • arrested B cell differentiation / MGI
  • decreased IgG level / MGI
  • decreased IgM level / MGI
  • decreased IgA level / MGI
  • thymus hypoplasia / MGI
  • arrested T cell differentiation / MGI
  • lung inflammation / MGI
  • neoplasm / MGI
  • abnormal B cell differentiation / MGI
  • no abnormal phenotype detected / MGI
  • small lymph nodes / MGI
  • abnormal lymph node morphology / MGI
  • abnormal lymphopoiesis / MGI
  • abnormal pre-B cell morphology / MGI
  • increased susceptibility to infection / MGI
  • abnormal double-negative T cell morphology / MGI
  • abnormal double-positive T cell morphology / MGI
  • increased susceptibility to bacterial infection / MGI
  • abnormal effector T cell morphology / MGI
  • abnormal macrophage physiology / MGI
  • abnormal B cell number / MGI
  • abnormal B cell physiology / MGI
  • abnormal immune system organ morphology / MGI
  • colitis / MGI
  • absent Peyer's patches / MGI
  • increased natural killer cell mediated cytotoxicity / MGI
  • erythroderma / MGI
  • abnormal lymphocyte physiology / MGI
  • abnormal B cell morphology / MGI
  • decreased lymphocyte cell number / MGI
  • decreased B cell number / MGI
  • decreased T cell number / MGI
  • decreased susceptibility to parasitic infection / MGI
  • decreased double-negative T cell number / MGI
  • increased double-negative T cell number / MGI
  • decreased double-positive T cell number / MGI
  • decreased T cell proliferation / MGI
  • cachexia / MGI
  • increased macrophage cell number / MGI
  • abnormal T-helper 1 physiology / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • abnormal response to transplant / MGI
  • abnormal T cell morphology / MGI
  • decreased NK T cell number / MGI
  • decreased CD4-positive, alpha beta T cell number / MGI
  • decreased CD8-positive, alpha-beta T cell number / MGI
  • lymph node hypoplasia / MGI
  • decreased pre-B cell number / MGI
  • decreased mature B cell number / MGI
  • absent mature B cells / MGI
  • increased immature B cell number / MGI
  • decreased immature B cell number / MGI
  • abnormal gamma-delta T cell differentiation / MGI
  • decreased spleen white pulp amount / MGI
  • abnormal interferon secretion / MGI
  • abnormal chemokine secretion / MGI
  • abnormal T cell receptor V(D)J recombination / MGI
  • abnormal immunoglobulin V(D)J recombination / MGI
  • absent Hassall's corpuscle / MGI
  • small inguinal lymph nodes / MGI
  • decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
  • increased DN3 thymocyte number / MGI
  • abnormal stomach mucosa morphology / MGI
  • small cervical lymph nodes / MGI

Literature references

  • A role for CD40 expression on CD8+ T cells in the generation of CD8+ T cell memory.;Bourgeois Christine, Rocha Benedita, Tanchot Corinne, ;2002;Science (New York, N.Y.);297;2060-3; 12242444

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

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Genotyping protocol

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EMMA conditions
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