IMPC phenotypes (gene matching)
STOCK Vav2tm1Kdf Vav1tm2Bbd/Cnbc
| Status | Available to order |
| EMMA ID | EM:08030 |
| International strain name | STOCK Vav2tm1Kdf Vav1tm2Bbd/Cnbc |
| Alternative name | Double KO: vav1_KO; vav2_KO or Vav1tm2Bbd; Vav2tm1Kdf |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Vav1tm2Bbd, Vav2tm1Kdf |
| Gene/Transgene symbol | Vav1, Vav2 |
Information from provider
| Provider | Mariano Barbacid |
| Provider affiliation | Molecular Oncology, Centro Nacional de Investigaciones Oncologicas |
| Genetic information | In the Vav1 allele sequences encoding amino acids 135-195 comprising the entire acidic domain were replaced with a PGK-neo cassette via homologous recombination. Gene expression was absent in thymocytes of homozygous mutant animals. In the Vav2 allele a lacZ-neo cassette was fused in-frame to amino acids 150-155 of exon 5 and introduced via homologous recombination. Western blot analysis of spleen tissue from homozygous mutant animals confirmed the absence of gene expression. |
| Phenotypic information | Homozygous:Combined deletion of both Vav1 and Vav2 in mice results in a marked reduction in mature B lymphocyte numbers. Vav1(-/-),Vav-2(-/-) B cells are unresponsive to B cell antigen receptor (BCR)-driven proliferation in vitro and to thymus-independent antigen in vivo. BCR-stimulated intracellular calcium mobilization is greatly impaired in Vav1(-/-),Vav-2(-/-) B cells.Heterozygous:Heterozygous mice are viable |
| Breeding history | Chimeras were crossed with CD1 females for germ-line transmission analysis. Heterozygous animals for each allele were crossed among themselves to generate corresponding homozygous animals. To obtain the double mutant strain homozygous animals for each allele were crossbred. Currently maintained as mixed CD1 and 129. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous CD-1 outbred stock (syn.: outbr. CD-1 or CD1, Swiss CD-1 or CD1, ICR(CD-1), etc.), homozygous CD-1 outbred stock (syn.: outbr. CD-1 or CD1, Swiss CD-1 or CD1, ICR(CD-1), etc.) |
| Stage of embryos | 4/8-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
- abnormal T cell physiology / MGI
- abnormal positive T cell selection / MGI
- decreased T cell number / MGI
- impaired natural killer cell mediated cytotoxicity / MGI
- decreased double-positive T cell number / MGI
- immune system phenotype / MGI
- decreased NK T cell number / MGI
- abnormal CD4-positive T cell differentiation / MGI
- decreased interleukin-4 secretion / MGI
- decreased IgG level / MGI
- abnormal T cell differentiation / MGI
- abnormal B cell number / MGI
- decreased B-1 B cell number / MGI
- abnormal response to infection / MGI
- increased double-negative T cell number / MGI
- decreased B cell proliferation / MGI
- abnormal T cell number / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- increased transitional stage B cell number / MGI
- decreased IgG1 level / MGI
- decreased IgG2a level / MGI
- decreased IgG3 level / MGI
- abnormal splenic cell ratio / MGI
- abnormal lymph node cell ratio / MGI
- thymus hypoplasia / MGI
- abnormal T cell activation / MGI
- decreased T cell proliferation / MGI
- decreased interleukin-2 secretion / MGI
- abnormal iridocorneal angle / MGI
- ocular hypertension / MGI
- anterior iris synechia / MGI
- decreased mature B cell number / MGI
MGI phenotypes (gene matching)
- decreased thymocyte number / MGI
- abnormal lymphocyte cell number / MGI
- abnormal immune system physiology / MGI
- abnormal humoral immune response / MGI
- decreased IgG level / MGI
- thymus hypoplasia / MGI
- abnormal T cell activation / MGI
- abnormal T cell differentiation / MGI
- no abnormal phenotype detected / MGI
- abnormal double-negative T cell morphology / MGI
- abnormal CD4-positive, alpha beta T cell morphology / MGI
- abnormal T cell physiology / MGI
- abnormal B cell number / MGI
- decreased IgD level / MGI
- increased IgM level / MGI
- abnormal immune serum protein physiology / MGI
- failure of blastocyst to hatch from the zona pellucida / MGI
- transmission ratio distortion / MGI
- abnormal CD8-positive, alpha-beta T cell physiology / MGI
- abnormal negative T cell selection / MGI
- abnormal positive T cell selection / MGI
- decreased B-1 B cell number / MGI
- increased T cell number / MGI
- decreased T cell number / MGI
- abnormal response to infection / MGI
- impaired natural killer cell mediated cytotoxicity / MGI
- decreased double-negative T cell number / MGI
- increased double-negative T cell number / MGI
- decreased double-positive T cell number / MGI
- decreased B cell proliferation / MGI
- decreased T cell proliferation / MGI
- immune system phenotype / MGI
- abnormal CD4-positive, alpha-beta T cell physiology / MGI
- abnormal T cell number / MGI
- decreased NK T cell number / MGI
- decreased CD4-positive, alpha beta T cell number / MGI
- abnormal CD4-positive T cell differentiation / MGI
- decreased CD8-positive, alpha-beta T cell number / MGI
- decreased single-positive T cell number / MGI
- increased transitional stage B cell number / MGI
- decreased mature B cell number / MGI
- decreased IgG1 level / MGI
- decreased IgG2a level / MGI
- decreased IgG3 level / MGI
- decreased tumor necrosis factor secretion / MGI
- decreased interferon-gamma secretion / MGI
- increased circulating interferon-gamma level / MGI
- abnormal interleukin-2 secretion / MGI
- decreased interleukin-2 secretion / MGI
- decreased interleukin-4 secretion / MGI
- abnormal splenic cell ratio / MGI
- abnormal lymph node cell ratio / MGI
- increased DN2 thymocyte number / MGI
- increased DN3 thymocyte number / MGI
- increased central memory CD4-positive, alpha-beta T cell number / MGI
- increased effector memory CD4-positive, alpha-beta T cell number / MGI
- increased central memory CD8 positive, alpha-beta T cell number / MGI
- embryonic lethality between implantation and somite formation, complete penetrance / MGI
- decreased CD8-positive, naive alpha-beta T cell number / MGI
- hypertension / MGI
- abnormal heart morphology / MGI
- abnormal adrenal gland secretion / MGI
- decreased IgM level / MGI
- hydroencephaly / MGI
- heart left ventricle hypertrophy / MGI
- increased heart rate / MGI
- increased circulating aldosterone level / MGI
- cardiac fibrosis / MGI
- increased circulating renin level / MGI
- oliguria / MGI
- renal fibrosis / MGI
- abnormal iridocorneal angle / MGI
- enlarged myocardial fiber / MGI
- abnormal class switch recombination / MGI
- increased mean systemic arterial blood pressure / MGI
- decreased B cell number / MGI
- decreased B cell proliferation / MGI
- ocular hypertension / MGI
- vascular smooth muscle hyperplasia / MGI
- decreased creatinine clearance / MGI
- increased renin activity / MGI
- increased circulating antidiuretic hormone level / MGI
- increased circulating adrenaline level / MGI
- increased circulating noradrenaline level / MGI
- decreased urine sodium level / MGI
- decreased mature B cell number / MGI
- enlarged adrenal medulla / MGI
- adrenal medulla hyperplasia / MGI
- decreased spleen germinal center number / MGI
- decreased IgG1 level / MGI
- decreased IgG2b level / MGI
- decreased IgG3 level / MGI
- abnormal physiological response to xenobiotic / MGI
- abnormal bradykinin level / MGI
- abnormal aorta tunica media morphology / MGI
- anterior iris synechia / MGI
Literature references
- Vav is a regulator of cytoskeletal reorganization mediated by the T-cell receptor.;Fischer K D, Kong Y Y, Nishina H, Tedford K, Marengère L E, Kozieradzki I, Sasaki T, Starr M, Chan G, Gardener S, Nghiem M P, Bouchard D, Barbacid M, Bernstein A, Penninger J M, ;1998;Current biology : CB;8;554-62; 9601639
- Compensation between Vav-1 and Vav-2 in B cell development and antigen receptor signaling.;Tedford K, Nitschke L, Girkontaite I, Charlesworth A, Chan G, Sakk V, Barbacid M, Fischer K D, ;2001;Nature immunology;2;548-55; 11376343