129P2/OlaHsd-Galtm1Wyn/WynH

Status

Available to order

EMMA IDEM:08194
International strain name129P2/OlaHsd-Galtm1Wyn/WynH
Alternative nameGalanin KO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolGaltm1Wyn
Gene/Transgene symbolGal

Information from provider

ProviderDavid Wynick
Provider affiliationMedical Sciences Bristol, Research NBT and UHBristol
Genetic informationLoss of function mutation in the galanin gene.
Phenotypic informationHomozygous:
Developmental deficits in dorsal root ganglion (DRG) and cholinergic neurons, absence of neuropathic pain, deficits in neuronal regeneration and neurite outgrowth and more severe disease in experimental autoimmune encephalomyelitis (EAE) model of multiple sclerosis (MS) and A-beta model of Alzheimer's disease (AD).

Heterozygous:
No phenotype.
Breeding historyE14 ES cell line - maintained on pure 129P2/OlaHsd
References
  • A role for galanin in human and experimental inflammatory demyelination.;Wraith David C, Pope Robert, Butzkueven Helmut, Holder Heidi, Vanderplank Penny, Lowrey Pauline, Day Michael J, Gundlach Andrew L, Kilpatrick Trevor J, Scolding Neil, Wynick David, ;2009;Proceedings of the National Academy of Sciences of the United States of America;106;15466-71; 19717462
  • Feeding behaviour in galanin knockout mice supports a role of galanin in fat intake and preference.;Adams A C, Clapham J C, Wynick D, Speakman J R, ;2008;Journal of neuroendocrinology;20;199-206; 18088361
  • Loss-of-function mutation of the galanin gene is associated with perturbed islet function in mice.;Ahrén Bo, Pacini Giovanni, Wynick David, Wierup Nils, Sundler Frank, ;2004;Endocrinology;145;3190-6; 15044363
  • Galanin acts as a neuroprotective factor to the hippocampus.;Elliott-Hunt Caroline R, Marsh Barnaby, Bacon Andrea, Pope Robert, Vanderplank Penny, Wynick David, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;5105-10; 15041741
  • The second galanin receptor GalR2 plays a key role in neurite outgrowth from adult sensory neurons.;Mahoney Sally-Ann, Hosking Richard, Farrant Sarah, Holmes Fiona E, Jacoby Arie S, Shine John, Iismaa Tiina P, Scott Malcolm K, Schmidt Ralf, Wynick David, ;2003;The Journal of neuroscience : the official journal of the Society for Neuroscience;23;416-21; 12533601
  • Endogenous galanin is required for the full expression of central sensitization following peripheral nerve injury.;Kerr B J, Thompson S W, Wynick D, McMahon S B, ;2001;Neuroreport;12;3331-4; 11711881
  • Targeted disruption of the galanin gene reduces the number of sensory neurons and their regenerative capacity.;Holmes F E, Mahoney S, King V R, Bacon A, Kerr N C, Pachnis V, Curtis R, Priestley J V, Wynick D, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;11563-8; 11016970
  • Galanin knockout mice reveal nociceptive deficits following peripheral nerve injury.;Kerr B J, Cafferty W B, Gupta Y K, Bacon A, Wynick D, McMahon S B, Thompson S W, ;2000;The European journal of neuroscience;12;793-802; 10762308
  • Modulation of hippocampal excitability and seizures by galanin.;Mazarati A M, Hohmann J G, Bacon A, Liu H, Sankar R, Steiner R A, Wynick D, Wasterlain C G, ;2000;The Journal of neuroscience : the official journal of the Society for Neuroscience;20;6276-81; 10934278
  • Galanin regulates the postnatal survival of a subset of basal forebrain cholinergic neurons.;O'Meara G, Coumis U, Ma S Y, Kehr J, Mahoney S, Bacon A, Allen S J, Holmes F, Kahl U, Wang F H, Kearns I R, Ove-Ogren S, Dawbarn D, Mufson E J, Davies C, Dawson G, Wynick D, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;11569-74; 11016971
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreMary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal branching of the mammary ductal tree / MGI
  • abnormal hippocampus morphology / MGI
  • decreased sensory neuron number / MGI
  • small L4 dorsal root ganglion / MGI
  • small L5 dorsal root ganglion / MGI
  • abnormal object recognition memory / MGI
  • abnormal circulating insulin level / MGI
  • abnormal pituitary secretion / MGI
  • abnormal lactation / MGI
  • seizures / MGI
  • abnormal gland morphology / MGI
  • abnormal gland physiology / MGI
  • increased susceptibility to pharmacologically induced seizures / MGI
  • enhanced long term potentiation / MGI
  • decreased insulin secretion / MGI
  • abnormal nervous system regeneration / MGI
  • increased susceptibility to neuronal excitotoxicity / MGI
  • decreased lactotroph cell number / MGI
  • lactation failure / MGI
  • weight loss / MGI
  • abnormal testes secretion / MGI
  • enlarged seminal vesicle / MGI
  • decreased circulating glucocorticoid level / MGI
  • decreased testis weight / MGI
MGI phenotypes (gene matching)
  • abnormal branching of the mammary ductal tree / MGI
  • abnormal hippocampus morphology / MGI
  • decreased sensory neuron number / MGI
  • small L4 dorsal root ganglion / MGI
  • small L5 dorsal root ganglion / MGI
  • weight loss / MGI
  • abnormal object recognition memory / MGI
  • abnormal circulating insulin level / MGI
  • abnormal pituitary secretion / MGI
  • abnormal lactation / MGI
  • seizures / MGI
  • abnormal gland morphology / MGI
  • abnormal gland physiology / MGI
  • abnormal testes secretion / MGI
  • increased susceptibility to pharmacologically induced seizures / MGI
  • enlarged seminal vesicle / MGI
  • enhanced long term potentiation / MGI
  • decreased insulin secretion / MGI
  • decreased circulating glucocorticoid level / MGI
  • decreased testis weight / MGI
  • abnormal nervous system regeneration / MGI
  • increased susceptibility to neuronal excitotoxicity / MGI
  • decreased lactotroph cell number / MGI
  • lactation failure / MGI

Literature references

  • A role for galanin in human and experimental inflammatory demyelination.;Wraith David C, Pope Robert, Butzkueven Helmut, Holder Heidi, Vanderplank Penny, Lowrey Pauline, Day Michael J, Gundlach Andrew L, Kilpatrick Trevor J, Scolding Neil, Wynick David, ;2009;Proceedings of the National Academy of Sciences of the United States of America;106;15466-71; 19717462
  • Feeding behaviour in galanin knockout mice supports a role of galanin in fat intake and preference.;Adams A C, Clapham J C, Wynick D, Speakman J R, ;2008;Journal of neuroendocrinology;20;199-206; 18088361
  • Loss-of-function mutation of the galanin gene is associated with perturbed islet function in mice.;Ahrén Bo, Pacini Giovanni, Wynick David, Wierup Nils, Sundler Frank, ;2004;Endocrinology;145;3190-6; 15044363
  • Galanin acts as a neuroprotective factor to the hippocampus.;Elliott-Hunt Caroline R, Marsh Barnaby, Bacon Andrea, Pope Robert, Vanderplank Penny, Wynick David, ;2004;Proceedings of the National Academy of Sciences of the United States of America;101;5105-10; 15041741
  • The second galanin receptor GalR2 plays a key role in neurite outgrowth from adult sensory neurons.;Mahoney Sally-Ann, Hosking Richard, Farrant Sarah, Holmes Fiona E, Jacoby Arie S, Shine John, Iismaa Tiina P, Scott Malcolm K, Schmidt Ralf, Wynick David, ;2003;The Journal of neuroscience : the official journal of the Society for Neuroscience;23;416-21; 12533601
  • Endogenous galanin is required for the full expression of central sensitization following peripheral nerve injury.;Kerr B J, Thompson S W, Wynick D, McMahon S B, ;2001;Neuroreport;12;3331-4; 11711881
  • Targeted disruption of the galanin gene reduces the number of sensory neurons and their regenerative capacity.;Holmes F E, Mahoney S, King V R, Bacon A, Kerr N C, Pachnis V, Curtis R, Priestley J V, Wynick D, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;11563-8; 11016970
  • Galanin knockout mice reveal nociceptive deficits following peripheral nerve injury.;Kerr B J, Cafferty W B, Gupta Y K, Bacon A, Wynick D, McMahon S B, Thompson S W, ;2000;The European journal of neuroscience;12;793-802; 10762308
  • Modulation of hippocampal excitability and seizures by galanin.;Mazarati A M, Hohmann J G, Bacon A, Liu H, Sankar R, Steiner R A, Wynick D, Wasterlain C G, ;2000;The Journal of neuroscience : the official journal of the Society for Neuroscience;20;6276-81; 10934278
  • Galanin regulates the postnatal survival of a subset of basal forebrain cholinergic neurons.;O'Meara G, Coumis U, Ma S Y, Kehr J, Mahoney S, Bacon A, Allen S J, Holmes F, Kahl U, Wang F H, Kearns I R, Ove-Ogren S, Dawbarn D, Mufson E J, Davies C, Dawson G, Wynick D, ;2000;Proceedings of the National Academy of Sciences of the United States of America;97;11569-74; 11016971

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*
  • Tissue - Types of tissue, service fee and delivery time available upon request

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