IMPC phenotypes (gene matching)
B6NCrl.129P2(Cg)-Tyk2tm3.1Biat/Biat
| Status | Available to order |
| EMMA ID | EM:09905 |
| International strain name | B6NCrl.129P2(Cg)-Tyk2tm3.1Biat/Biat |
| Alternative name | B6NCrl.129P2(C)-Tyk2 |
| Strain type | Targeted Mutant Strains : Point mutation |
| Allele/Transgene symbol | Tyk2tm3.1Biat |
| Gene/Transgene symbol | Tyk2 |
Information from provider
| Provider | Mathias Müller |
| Provider affiliation | Department for Biomedical Sciences, University of Veterinary Medicine Vienna (Vetmeduni Vienna) |
| Additional owner | Prof. Thomas Rülicke, University of Veterinary Medicine, Institute of Laboratory Animal Science, Vienna, Austria |
| Genetic information | Exon 20 was replaced with a modified one in which nucleotide substitutions (AAG GCC CTG to GAG GCC CTT) results in the amino acid substitution of glutamic acid for lysine at position 923 (K923E) and introduced a BspTI restriction enzyme site. A floxed neo cassette inserted downstream of exon 21 was removed by cre-mediated recombination. In vitro kinase activity confirmed the absence of activity. (PubMed ID:22723949) |
| Phenotypic information | Homozygous:immune defectsHeterozygous:none |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | yes |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
Disease and phenotype information
MGI phenotypes (allele matching)
- increased susceptibility to viral infection induced morbidity/mortality / MGI
MGI phenotypes (gene matching)
- increased body weight / MGI
- obese / MGI
- increased circulating free fatty acid level / MGI
- abnormal body temperature homeostasis / MGI
- decreased IgG level / MGI
- increased circulating insulin level / MGI
- abnormal T cell differentiation / MGI
- increased susceptibility to viral infection / MGI
- abnormal T cell physiology / MGI
- abnormal brown adipose tissue morphology / MGI
- abnormal cell cycle / MGI
- decreased susceptibility to induced arthritis / MGI
- abnormal nitric oxide homeostasis / MGI
- increased cholesterol level / MGI
- decreased energy expenditure / MGI
- abnormal response to infection / MGI
- increased susceptibility to parasitic infection / MGI
- impaired glucose tolerance / MGI
- abnormal T-helper 1 physiology / MGI
- abnormal mitochondrial physiology / MGI
- abnormal common myeloid progenitor cell morphology / MGI
- decreased IgG1 level / MGI
- decreased IgG2a level / MGI
- decreased interferon-gamma secretion / MGI
- increased circulating interleukin-12 level / MGI
- abnormal splenocyte physiology / MGI
- increased susceptibility to viral infection induced morbidity/mortality / MGI
- impaired adaptive thermogenesis / MGI
- decreased macrophage nitric oxide production / MGI
- abnormal fat cell differentiation / MGI
- disorganized mitochondrial cristae / MGI
Literature references
- TYK2 kinase activity is required for functional type I interferon responses in vivo.;Prchal-Murphy Michaela, Semper Christian, Lassnig Caroline, Wallner Barbara, Gausterer Christian, Teppner-Klymiuk Ingeborg, Kobolak Julianna, Müller Simone, Kolbe Thomas, Karaghiosoff Marina, Dinnyés Andras, Rülicke Thomas, Leitner Nicole R, Strobl Birgit, Müller Mathias, ;2012;PloS one;7;e39141; 22723949