B6.Cg-Tg(ACTA2-TFPI/CD4/SELP)#aAdor/AdorH
| Status | Available to order |
| EMMA ID | EM:08451 |
| Citation information | RRID:IMSR_EM:08451 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.Cg-Tg(ACTA2-TFPI/CD4/SELP)#aAdor/AdorH |
| Alternative name | alpha-TFPI-Tg |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(ACTA2-TFPI/CD4/SELP)#aAdor |
| Gene/Transgene symbol | Tg(ACTA2-TFPI/CD4/SELP)#aAdor |
Information from provider
| Provider | Anthony Dorling |
| Provider affiliation | Innate Immunity, King's College London |
| Additional owner | Mark Thursz, Imperial College London, UK and Li Ke, Xi'an Jiatong University, China |
| Genetic information | Human tissue factor pathway inhibitor/CD4/P-selectin construct, with a modified alpha-smooth muscle actin promoter, introduced by microinjection. Copy number originally >30. |
| Phenotypic information | Homozygous:Mice express membrane tethered human TFPI-CD4 fusion protein on all alpha-SMA+ cells. In cells with secretory granules, these will be internalised at resting state by virtue of P-selectin cytoplasmic sequence. The hTFPI inhibits tissue factor and FXa.Heterozygous:Mice express membrane tethered human TFPI-CD4 fusion protein on all alpha-SMA+ cells. In cells with secretory granules, these will be internalised at resting state by virtue of P-selectin cytoplasmic sequence. The hTFPI inhibits tissue factor and FXa. |
| Breeding history | Started backcrossing onto C57BL/6 in 2003, and then bred as sibling pairs since 2009. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- Human tissue factor pathway inhibitor fused to CD4 binds both FXa and TF/FVIIa at the cell surface.;Riesbeck K, Dorling A, Kemball-Cook G, McVey J H, Jones M, Tuddenham E G, Lechler R I, ;1997;Thrombosis and haemostasis;78;1488-94; 9423800
- Postinjury vascular intimal hyperplasia in mice is completely inhibited by CD34+ bone marrow-derived progenitor cells expressing membrane-tethered anticoagulant fusion proteins.;Chen D, Weber M, Shiels P G, Dong R, Webster Z, McVey J H, Kemball-Cook G, Tuddenham E G D, Lechler R I, Dorling A, ;2006;Journal of thrombosis and haemostasis : JTH;4;2191-8; 16803463
- Inhibition of thrombin receptor signaling on α-smooth muscle actin(+) CD34(+) progenitors leads to repair after murine immune vascular injury.;Chen Daxin, Shrivastava Seema, Ma Liang, Tham El-Li, Abrahams Joel, Coe J David, Scott Diane, Lechler Robert I, McVey John H, Dorling Anthony, ;2012;Arteriosclerosis, thrombosis, and vascular biology;32;42-9; 22034512
- Fibrocytes mediate intimal hyperplasia post-vascular injury and are regulated by two tissue factor-dependent mechanisms.;Chen D, Ma L, Tham E-L, Maresh S, Lechler R I, McVey J H, Dorling A, ;2013;Journal of thrombosis and haemostasis : JTH;11;963-74; 23516969
- Regulated inhibition of coagulation by porcine endothelial cells expressing P-selectin-tagged hirudin and tissue factor pathway inhibitor fusion proteins.;Chen D, Riesbeck K, McVey J H, Kemball-Cook G, Tuddenham E G, Lechler R I, Dorling A, ;1999;Transplantation;68;832-9; 10515384
- Inhibition of tissue factor-dependent and -independent coagulation by cell surface expression of novel anticoagulant fusion proteins.;Chen D, Riesbeck K, Kemball-Cook G, McVey J H, Tuddenham E G, Lechler R I, Dorling A, ;1999;Transplantation;67;467-74; 10030296