C57BL/6J-Tg(Gad2-tdTomato)DJhi/Ph
| Status | Available to order |
| EMMA ID | EM:10422 |
| International strain name | C57BL/6J-Tg(Gad2-tdTomato)DJhi/Ph |
| Alternative name | B6-Tg(Gad2-tdTomato)D |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gad2-tdTomato)DJhi, |
| Gene/Transgene symbol | Tg(Gad2-tdTomato)DJhi |
Information from provider
| Provider | Johannes Hirrlinger |
| Provider affiliation | Carl-Ludwig-Institute for Physiology, University of Leipzig |
| Genetic information | The BAC clone 407K8 from the mouse C57BL/6 BAC library RPCI-23 (Research Genetics Inc., Huntsville, AL) containing the GAD2 (GAD65) gene was modified by inserting the tdTomato-ORF after the endogenous start signal. The modified BAC-GAD65-tdTomato construct was integrated into an unknown locus of C57BL/6 mice. |
| Phenotypic information | Homozygous:Transgenic mice express tdTomato in 80-90% of GABAergic, GAD65-positive cells. Fluorescent signals were observed in the olfactory bulb, striatum, cortex, hippocampus, cerebellum, brainstem and spinal cord as well as retinal ganglion and amacrine cells.Heterozygous:Should be same as for the homozygous animals. However, mouse producer does only test for presence of transgene and not for zygosity. |
| Breeding history | Transgenic founder mice were originally bred to C57BL/6JOlaHsd mice. Later mice were bred to mice from the transgenic line itself. Mice were bred for about 6 generations so far. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institute of Molecular Genetics, Prague, Czech Republic |
| Animals used for archiving | heterozygous 0 |
Literature references
- A Transgenic Mouse Line Expressing the Red Fluorescent Protein tdTomato in GABAergic Neurons.;Besser Stefanie, Sicker Marit, Marx Grit, Winkler Ulrike, Eulenburg Volker, Hülsmann Swen, Hirrlinger Johannes, ;2015;PloS one;10;e0129934; 26076353