IMPC phenotypes (gene matching)
B6;129P2-Hprt1tm1(CAG-EGFP)Laumm/H
| Status | Available to order |
| EMMA ID | EM:12427 |
| Citation information | RRID:IMSR_EM:12427 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6;129P2-Hprt1tm1(CAG-EGFP)Laumm/H |
| Alternative name | LifeAct-GFP |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Hprt1tm1(CAG-EGFP)Laumm |
| Gene/Transgene symbol | Hprt1 |
Information from provider
| Provider | Laura Machesky |
| Provider affiliation | The Beatson Institute for Cancer Research |
| Additional owner | LifecAct plasmid from Ibidi GFP patent owned by GE Healthcare, due to expire in Sept 2021 |
| Genetic information | The cre recombinase-inducible transgenic mice can conditionally express cell-type specific LifeAct-GFP. This is possible due to a floxed neomycin resistance 'STOP' cassette encoded upstream of the LifeAct-GFP fusion cDNA in the Hprt1 locus of the mouse X chromosome. |
| Phenotypic information | Homozygous:No overt phenotype. LifeAct is a 17 amino-acid peptide, isolated from yeast, which facilitates visualisation of filamentous actin (F-actin) in cells and tissues without significantly interfering with actin dynamics. This enables live imaging of the actin cytoskeleton.Heterozygous:No overt phenotype. LifeAct is a 17 amino-acid peptide, isolated from yeast, which facilitates visualisation of filamentous actin (F-actin) in cells and tissues without significantly interfering with actin dynamics. This enables live imaging of the actin cytoskeleton. |
| Breeding history | Intercrossing of LifeAct-GFP mice yielded the expected Mendelian ratios, indicating that no embryonic lethality had occurred. Moreover, LifeAct-GFP male and female mice are fertile and have no major defects in any organs studied. Animals are reproductively mature at 8 weeks of age and a decline in reproductivity can be observed from 6 months of age. Average length of gestation is 19 days. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI phenotypes (gene matching)
- decreased hematocrit / MGI
- increased leukocyte cell number / MGI
- increased neutrophil cell number / MGI
- abnormal small intestine morphology / MGI
- abnormal liver morphology / MGI
- abnormal branching of the mammary ductal tree / MGI
- enlarged spleen / MGI
- spleen hyperplasia / MGI
- enlarged lymph nodes / MGI
- tremors / MGI
- convulsive seizures / MGI
- abnormal lung morphology / MGI
- decreased body weight / MGI
- decreased anxiety-related response / MGI
- ataxia / MGI
- hypoactivity / MGI
- impaired coordination / MGI
- abnormal gait / MGI
- short stride length / MGI
- decreased exploration in new environment / MGI
- limb grasping / MGI
- abnormal motor coordination/balance / MGI
- abnormal hematopoietic system physiology / MGI
- hyperglycemia / MGI
- anemia / MGI
- cardiac hypertrophy / MGI
- increased mammary adenocarcinoma incidence / MGI
- abnormal reflex / MGI
- seizures / MGI
- abnormal motor capabilities/coordination/movement / MGI
- premature death / MGI
- abnormal definitive hematopoiesis / MGI
- abnormal brain morphology / MGI
- no abnormal phenotype detected / MGI
- neurodegeneration / MGI
- abnormal spleen white pulp morphology / MGI
- abnormal hematopoietic system morphology/development / MGI
- abnormal megakaryocyte progenitor cell morphology / MGI
- hepatic steatosis / MGI
- decreased vertical activity / MGI
- increased heart weight / MGI
- increased systemic arterial blood pressure / MGI
- albuminuria / MGI
- decreased erythrocyte cell number / MGI
- increased urine protein level / MGI
- impaired social transmission of food preference / MGI
- no phenotypic analysis / MGI
- phenotypic reversion / MGI
- abnormal dopaminergic neuron morphology / MGI
- astrocytosis / MGI
- abnormal depression-related behavior / MGI
- decreased tumor growth/size / MGI
- abnormal nervous system morphology / MGI
- abnormal cardiac muscle relaxation / MGI
- neuronal intranuclear inclusions / MGI
- abnormal myocardial fiber physiology / MGI
- abnormal Paneth cell morphology / MGI
- decreased B cell number / MGI
- decreased cardiac muscle contractility / MGI
- glomerulosclerosis / MGI
- abnormal podocyte morphology / MGI
- muscle phenotype / MGI
- homeostasis/metabolism phenotype / MGI
- endocrine/exocrine gland phenotype / MGI
- behavior/neurological phenotype / MGI
- immune system phenotype / MGI
- taste/olfaction phenotype / MGI
- hematopoietic system phenotype / MGI
- jerky movement / MGI
- thrombocytosis / MGI
- decreased ventricle muscle contractility / MGI
- decreased mean corpuscular hemoglobin concentration / MGI
- decreased dopamine level / MGI
- abnormal podocyte slit diaphragm morphology / MGI
- absent podocyte slit diaphragm / MGI
- podocyte foot process effacement / MGI
- increased megakaryocyte cell number / MGI
- abnormal spatial reference memory / MGI
- abnormal spatial working memory / MGI
- abnormal splenic cell ratio / MGI
- abnormal physiological response to xenobiotic / MGI
- abnormal enterocyte proliferation / MGI
- abnormal enterocyte apoptosis / MGI
- abnormal neuron differentiation / MGI
- increased mammary gland tumor incidence / MGI
- myeloid hyperplasia / MGI
- expanded mesangial matrix / MGI
- mesangial cell hyperplasia / MGI
- abnormal habituation to a new environment / MGI
- abnormal ceramide level / MGI
- decreased brain choline acetyltransferase activity / MGI
- decreased brain tyrosine 3-monooxygenase activity / MGI
- decreased vascular endothelial cell proliferation / MGI
Literature references
- Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo.;Schachtner Hannah, Li Ang, Stevenson David, Calaminus Simon D J, Thomas Steve, Watson Steve P, Sixt Michael, Wedlich-Soldner Roland, Strathdee Douglas, Machesky Laura M, ;2012;European journal of cell biology;91;923-929; 22658956