Selected reading assisted reproductive technology (ART), gnotobiology and health monitoring

INFRAFRONTIER has collected publications covering different aspects of assisted reproductive technology (ART), gnotobiology and health monitoring. The EMMA partners find these articles and reviews useful for scientists who want to apply these techniques. The lists are not exhaustive.

Intracytoplasmic sperm injection (ICSI)

Key references

  • Fulka H, Fulka J, Jr. No differences in the DNA methylation pattern in mouse zygotes produced in vivo, in vitro, or by intracytoplasmic sperm injection. Fertil Steril 2006; 86: 1534-6. PMID: 16979637
  • Kawase Y, Aoki Y, Kamada N, Jishage K, Suzuki H. Comparison of fertility between intracytoplasmic sperm injection and in vitro fertilization with a partial zona pellucida incision by using a piezo-micromanipulator in cryopreserved inbred mouse spermatozoa. Contemp Top Lab Anim Sci 2004; 43: 21-5. PMID: 14984285
  • Kimura Y, Yanagimachi R. Intracytoplasmic sperm injection in the mouse. Biol Reprod 1995; 52: 709-20. PMID: 7779992
  • Lacham-Kaplan O, Shaw J, Sanchez-Partida LG, Trounson A. Oocyte activation after intracytoplasmic injection with sperm frozen without cryoprotectants results in live offspring from inbred and hybrid mouse strains. Biol Reprod 2003; 69: 1683-9. PMID: 12890731 
  • Raspa M, Mahabir E, Fray M, Volland R, Scavizzi F. Lack of transmission of murine norovirus to mice via in vitro fertilization, intracytoplasmic sperm injection, and ovary transplantation. Theriogenology 2016; 86:579-588. PMID: 26972226
  • Szczygiel MA, Kusakabe H, Yanagimachi R, Whittingham DG. Intracytoplasmic sperm injection is more efficient than in vitro fertilization for generating mouse embryos from cryopreserved spermatozoa. Biol Reprod 2002; 67: 1278-84. PMID: 12297546

More references

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Key references

  • Ley RE, Turnbaugh PJ, Klein S, Gordon JI. Microbiol ecology: human gut microbes associated with obesity. Nature 2006; 444: 1022-3. PMID: 17183309
  • Heijtz RD, Wang S, Anuar F, Qian Y, Björkholm B, Samuelsson A, Hibberd ML, Forssberg H, Pettersson S. Normal gut microbiota modulates brain development and behavior. Proc Natl Acad Sci U S A 2010; 108: 3047-52. PMID: 21282636
  • Mayer EA. Gut feelings: the emerging biology of gut-brain communication. Nat Rev Neurosci 2011; 12: 453-66. PMID: 21750565
Autoimmune diseases and allergy
  • Berer K, Mues M, Koutrolos M, Rasbi ZA, Boziki M, Johner C, Wekerle H, Krishnamoorthy G. Commensal microbiota and myelin autoantigen cooperate to trigger autoimmune demyelination. Nature 2011; 479: 538-41. PMID: 22031325
  • Hadis U, Wahl B, Schulz O, Hardtke-Wolenski M, Schippers A, Wagner N, Müller W, Sparwasser T, Förster R, Pabst O. Intestinal tolerance requires gut homing and expansion of FoxP3+ regulatory T cells in the lamina propria. Immunity 2011; 34: 237-46. PMID: 21333554
  • Wen L, Ley RE, Volchkov PY, Stranges PB, Avanesyan L, Stonebraker AC, Hu C, Wong FS, Szot GL, Bluestone JA, Gordon JI, Chervonsky AV. Innate immunity and intestinal microbiota in the development of Type 1 diabetes. Nature 2008; 455: 1109-13. PMID: 18806780
  • Kane M, Case LK, Kopaskie K, Kozlova A, MacDearmid C, Chervonsky AV, Golovkina TV. Successful transmission of a retrovirus depends on the commensal microbiota. Science 2011; 334: 245-9. PMID: 21998394
  • Kuss SK, Best GT, Etheredge CA, Pruijssers AJ, Frierson JM, Hooper LV, Dermody TS, Pfeiffer JK. Intestinal microbiota promote enteric virus replication and systemic pathogenesis. Science 2011; 334: 249-52. PMID: 21998395
Technical description on how to raise germ-free mice
  • Giraud A. Axenic mice model. Methods Mol Biol 2008; 415: 321-36. PMID: 18370163
Proxy for axenization: large spectrum antibiotics treatment
  • Rakoff-Nahoum S, Paglino J, Eslami-Varzaneh F, Edberg S, Medzhitov R. Recognition of commensal microflora by toll-like receptors is required for intestinal homeostasis. Cell 2004; 118: 229-41. PMID: 15260992
    This protocol for the treatment of mice with a cocktail of four antibiotics (ampicillin, neomycin, metronidazole and vancomycin) is often used as a pilot study before processing with full axenization.
Health monitoring

Key references

  • Recommendations for the health monitoring of mouse, rat, hamster, guineapig and rabbit breeding colonies. Report of the Federation of European Laboratory Animal Science Associations (FELASA) Working Group on Animal Healthaccepted by the FELASA Board of Management November 1992. Lab Anim 1994; 28: 1-12. PMID: 8158962
  • Agca Y, Bauer BA, Johnson DK, Critser JK, Riley LK. Detection of mouse parvovirus in Mus musculus gametes, embryos, and ovarian tissues by polymerase chain reaction assay. Comp Med 2007; 57: 51-6. PMID: 17348291
  • Forbes D. FELASA recommendations for the health monitoring of mouse, rat, hamster, guineapig and rabbit breeding colonies. Lab Anim 1994; 28: 279-80. PMID: 7967470
  • Hill AC, Stalley GP. Mycoplasma pulmonis infection with regard to embryo freezing and hysterectomy derivation. Lab Anim Sci 1991; 41: 563-6. PMID: 1667198
  • Kyuwa S, Nishikawa T, Kaneko T, Nakashima T, Kawano K, Nakamura N, et al. Experimental evaluation of cross-contamination between cryotubes containing mouse 2-cell embryos and murine pathogens in liquid nitrogen tanks. Exp Anim 2003; 52: 67-70. PMID: 12638239
  • Mahabir E, Bauer B, Schmidt J. Rodent and germplasm trafficking: risks of microbial contamination in a high-tech biomedical world. ILAR J 2008; 49: 347-355. PMID: 18506068
  • Rehbinder C, Baneux P, Forbes D, van Herck H, Nicklas W, Rugaya Z, et al. FELASA recommendations for the health monitoring of mouse, rat, hamster, gerbil, guinea pig and rabbit experimental units. Report of the Federation of European Laboratory Animal Science Associations (FELASA) Working Group on Animal Health accepted by the FELASA Board of Management, November 1995. Lab Anim 1996; 30: 193-208. PMID: 8843044