STOCK Cdc20tm1.1Mama/Cnbc

Status

Available to order

EMMA IDEM:06117
Citation informationRRID:IMSR_EM:06117 

Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.

International strain nameSTOCK Cdc20tm1.1Mama/Cnbc
Alternative nameCdc20
Strain typeTargeted Mutant Strains : Conditional mutation
Allele/Transgene symbolCdc20tm1.1Mama
Gene/Transgene symbolCdc20

Information from provider

ProviderMarcos Malumbres
Provider affiliationMolecular Oncology, Centro Nacional de Investigaciones Oncologicas (CNIO)
Genetic informationA loxP site was inserted upstream of exon 2, and an FRT-flanked neo cassette with a 5' loxP site was inserted downstream of exon 2; flp-mediated recombination removed the neo cassette leaving exon 2 floxed.
Phenotypic informationBefore cre-mediated recombination there is no phenotype associated to this allele.
Breeding historyChimeras were crossed with C57BL/6J females for germ line transmission testing. To remove the neoR selection marker Aurkb+/loxfrt mice were crossed with Tg(ACTFLPe)9205Dym mice to remove the frt-flanked neomycin resistance cassette used for homologous recombination selection. Currently maintained by crossing homozygous mice.
References
  • Targeting mitotic exit leads to tumor regression in vivo: Modulation by Cdk1, Mastl, and the PP2A/B55α,δ phosphatase.;Manchado Eusebio, Guillamot María, de Cárcer Guillermo, Eguren Manuel, Trickey Michelle, García-Higuera Irene, Moreno Sergio, Yamano Hiroyuki, Cañamero Marta, Malumbres Marcos, ;2010;Cancer cell;18;641-54; 21156286
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous males

Disease and phenotype information

IMPC phenotypes (gene matching)
  • increased heart weight / IMPC
  • preweaning lethality, complete penetrance / IMPC
  • embryonic lethality prior to tooth bud stage / IMPC
  • decreased circulating glucose level / IMPC
  • increased grip strength / IMPC
  • improved glucose tolerance / IMPC
  • increased fasting circulating glucose level / IMPC
  • increased circulating glucose level / IMPC
  • embryonic lethality prior to organogenesis / IMPC
MGI phenotypes (gene matching)
  • abnormal cell death / MGI
  • decreased cell proliferation / MGI
  • abnormal oocyte morphology / MGI
  • decreased embryo size / MGI
  • reduced female fertility / MGI
  • female infertility / MGI
  • increased tumor incidence / MGI
  • no phenotypic analysis / MGI
  • failure of zygotic cell division / MGI
  • abnormal chromosome morphology / MGI
  • maternal effect / MGI
  • aneuploidy / MGI
  • abnormal mitosis / MGI
  • abnormal female meiosis / MGI
  • reproductive system phenotype / MGI
  • abnormal male germ cell morphology / MGI
  • increased hepatoma incidence / MGI
  • embryonic lethality before implantation, complete penetrance / MGI
  • embryonic lethality between implantation and somite formation, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • embryonic lethality before implantation, incomplete penetrance / MGI
  • increased lymphoma incidence / MGI
  • increased embryonic tissue cell apoptosis / MGI

Literature references

  • Targeting mitotic exit leads to tumor regression in vivo: Modulation by Cdk1, Mastl, and the PP2A/B55α,δ phosphatase.;Manchado Eusebio, Guillamot María, de Cárcer Guillermo, Eguren Manuel, Trickey Michelle, García-Higuera Irene, Moreno Sergio, Yamano Hiroyuki, Cañamero Marta, Malumbres Marcos, ;2010;Cancer cell;18;641-54; 21156286

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

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Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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