STOCK Cdkn2atm2.1Rdp Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt

Status

Available to order

EMMA IDEM:04995
International strain nameSTOCK Cdkn2atm2.1Rdp Tg(Cnp-TVA,-lacZ)B8Ubc/Kctt
Alternative nameCtv-a INK4a-/-
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolCdkn2atm2.1Rdp,
Gene/Transgene symbolCdkn2a

Information from provider

ProviderLene Uhrbom
Provider affiliationDepartment of Neuroscience, Uppsala University
Additional ownerLene Uhrbom, Uppsala University, Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala, Sweden
Genetic informationTransgenic construct: pCNPase-TV-A-IRES-lacZ made in FVB/N. Crossed with strain containing targeted deletion p16INK4a (exon 1a of the cyclin-dependent kinase inhibitor 2A gene).
Phenotypic informationAllows for infection of 2',3'-cyclic nucleotide 3' phosphodiesterase (CNPase)-expressing cells by RCAS virus. The deletion of exon 1a in the cyclin-dependent kinase inhibitor 2A gene results in complete loss of p16INK4a transcription but not p19ARF.
Breeding historyThe TV-A founder mouse was crossed with FVB/N and subsequently intercrossed to establish homozygotes. Following, TVA-A transgenic mice were crossed with p16INK4a null mice and subsequently F1 progeny were crossed to generate mice homozygous for TV-A and p16INK4a deletion.
References
  • Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma.;Lindberg N, Kastemar M, Olofsson T, Smits A, Uhrbom L, ;2009;Oncogene;28;2266-75; 19421151
  • Loss of p16Ink4a with retention of p19Arf predisposes mice to tumorigenesis.;Sharpless N E, Bardeesy N, Lee K H, Carrasco D, Castrillon D H, Aguirre A J, Wu E A, Horner J W, DePinho R A, ;2001;Nature;413;86-91; 11544531
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivingheterozygous 0, wild-type FVB/N
Breeding at archiving centreNo breeding at Karolinska Inst.
Stage of embryos2-cell

Disease and phenotype information

MGI phenotypes (allele matching)
  • increased tumor incidence / MGI
  • thymus hyperplasia / MGI
  • increased sarcoma incidence / MGI
  • increased osteosarcoma incidence / MGI
  • increased incidence of tumors by chemical induction / MGI
  • increased thymus weight / MGI
  • increased double-positive T cell number / MGI
  • increased CD4-positive, alpha beta T cell number / MGI
  • increased CD8-positive, alpha-beta T cell number / MGI
  • increased small lymphocytic lymphoma incidence / MGI
  • increased splenocyte proliferation / MGI
  • increased lymphoma incidence / MGI

Literature references

  • Oligodendrocyte progenitor cells can act as cell of origin for experimental glioma.;Lindberg N, Kastemar M, Olofsson T, Smits A, Uhrbom L, ;2009;Oncogene;28;2266-75; 19421151
  • Loss of p16Ink4a with retention of p19Arf predisposes mice to tumorigenesis.;Sharpless N E, Bardeesy N, Lee K H, Carrasco D, Castrillon D H, Aguirre A J, Wu E A, Horner J W, DePinho R A, ;2001;Nature;413;86-91; 11544531

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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