STOCK Mir451atm1.2Doca/Cnrm
Status | Available to order |
EMMA ID | EM:05330 |
International strain name | STOCK Mir451atm1.2Doca/Cnrm |
Alternative name | Mir451 |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Mir451atm1.2Doca |
Gene/Transgene symbol | Mir451a |
Information from provider
Provider | Dónal O |
Provider affiliation | Mouse Biology Unit, European Molecular Biology Laboratory |
Genetic information | An FRT flanked neo cassette with a 5' loxP site was inserted downstream of the miR-144/451 locus. An additional loxP site was inserted between the two microRNAs. Flp mediated recombination removed the neo cassette leaving miR-451 floxed. Cre-mediated recombination deleted miR-451 within the miR-144/451 cluster, leaving expression of miR-144 intact. |
Phenotypic information | Mice deficient for the miR-451 display a cell autonomous impairment of late erythroblast maturation, resulting in erythroid hyperplasia, splenomegaly, and a mild anemia. |
Breeding history | Upon germ line transmission, the mice carrying the targeted allele were crossed to FLP-expressing transgenic mice (FLPeR) to remove the frt flanked neor cassette. The resulting mice were then crossed to CRE-expressing transgenic mice (Del-Cre) to delete miR-451. The mutant mice were backcrossed to C57BL/6N to remove the FLP and CRE transgenes. The offspring carrying the mutation were backcrossed 3 generations to C57BL/6N. |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (allele matching)
MGI phenotypes (gene matching)
- decreased hematocrit / MGI
- abnormal erythropoiesis / MGI
- enlarged spleen / MGI
- spleen hyperplasia / MGI
- anemia / MGI
- abnormal erythrocyte cell number / MGI
- abnormal hematopoietic system morphology/development / MGI
- decreased mean corpuscular volume / MGI
- reticulocytosis / MGI
- anisocytosis / MGI
- decreased hemoglobin content / MGI
- decreased erythrocyte cell number / MGI
- no phenotypic analysis / MGI
- increased erythroid progenitor cell number / MGI
- increased spleen weight / MGI
- polychromatophilia / MGI
- decreased mean corpuscular hemoglobin / MGI
- increased cellular sensitivity to hydrogen peroxide / MGI
- abnormal physiological response to xenobiotic / MGI
- increased physiological sensitivity to xenobiotic / MGI
- increased sensitivity to xenobiotic induced morbidity/mortality / MGI
- increased red blood cell distribution width / MGI
- increased number of Heinz bodies / MGI
- abnormal fetal derived definitive erythrocyte cell number / MGI
- decreased catalase activity / MGI
Literature references
- The miR-144/451 locus is required for erythroid homeostasis.;Rasmussen Kasper D, Simmini Salvatore, Abreu-Goodger Cei, Bartonicek Nenad, Di Giacomo Monica, Bilbao-Cortes Daniel, Horos Rastislav, Von Lindern Marieke, Enright Anton J, O'Carroll Dónal, ;2010;The Journal of experimental medicine;207;1351-8; 20513743
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