STOCK Otx2tm11Asim/Cnrm

Status

Available to order

EMMA IDEM:05624
International strain nameSTOCK Otx2tm11Asim/Cnrm
Alternative nameOtx2-GFP
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolOtx2tm11Asim,
Gene/Transgene symbolOtx2

Information from provider

ProviderAntonio Simeone
Provider affiliationCeinge Biotecnologie Avanzate
Genetic informationThe Otx2 coding sequence and introns are replaced with the GFP gene by homologous recombination.
Phenotypic informationBy 9.5 dpc, homozygous mutant embryos are characterized by the absence of forebrain and midbrain regions.
Breeding historyTargeted mutation was introduced in E14 ES cells. Mice were always maintained in a mixed C57BL/6 and DBA/2 genetic background.
References
  • Selective inactivation of Otx2 mRNA isoforms reveals isoform-specific requirement for visceral endoderm anteriorization and head morphogenesis and highlights cell diversity in the visceral endoderm.;Acampora Dario, Di Giovannantonio Luca Giovanni, Di Salvio Michela, Mancuso Pietro, Simeone Antonio, ;2009;Mechanisms of development;126;882-97; 19615442
Homozygous fertileno
Homozygous viableno
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • abnormal head morphology / MGI
  • abnormal embryo development / MGI
  • abnormal rod electrophysiology / MGI
  • abnormal cone electrophysiology / MGI
  • vision/eye phenotype / MGI
  • abnormal horizontal cell morphology / MGI
  • abnormal retinal bipolar cell morphology / MGI
  • decreased visual acuity / MGI
  • decreased retinal cone cell number / MGI
  • thin retinal inner nuclear layer / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • forebrain hypoplasia / MGI
  • neonatal lethality, complete penetrance / MGI
  • midbrain hypoplasia / MGI
  • abnormal forebrain development / MGI
  • abnormal midbrain development / MGI
  • acephaly / MGI

Literature references

  • Selective inactivation of Otx2 mRNA isoforms reveals isoform-specific requirement for visceral endoderm anteriorization and head morphogenesis and highlights cell diversity in the visceral endoderm.;Acampora Dario, Di Giovannantonio Luca Giovanni, Di Salvio Michela, Mancuso Pietro, Simeone Antonio, ;2009;Mechanisms of development;126;882-97; 19615442

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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