STOCK Tg(CAG-Katushka)3Sgo/Cnbc

Status

Available to order

EMMA IDEM:06790
International strain nameSTOCK Tg(CAG-Katushka)3Sgo/Cnbc
Alternative nameTg.CAG-LSL-KFP, Tg(CAG-Katushka)3Sgo
Strain typeTransgenic Strains
Allele/Transgene symbolTg(CAG-KFP)3Sgo
Gene/Transgene symbolTg(CAG-KFP)3Sgo

Information from provider

ProviderSagrario Ortega
Provider affiliationBiotechnology, Centro Nacional de Investigaciones Oncologicas
Genetic informationA 4.7 Kb DNA fragment of a plasmid containing the CAG promoter followed by a transcription/translation STOP sequence flanked by directly repeated loxP sites, the Katushka cDNA from pTurboFP635-C (Evrogen) and the rabbit O-globin polyadenylation signal, was injected into (B6 x CBA)F2 oocytes. This is a cre recombinase reporter line. After cre-mediated excision of the floxed STOP sequences the fluorescent Katushka protein is expressed under the control of the CAG ubiquitous promoter. This strain derives from founder #3.
Phenotypic informationMice do not have any significant phenotype.
Breeding historyCre-reporter founder animals were crossed with B6(Cg)-Tyrc-2J/J animals for testing germ line transmission. The line is maintained by crossing heterozygous animals with B6(Cg)-Tyrc-2J/J mice.
ReferencesNone available
Homozygous fertilenot known
Homozygous viablenot known
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain
Animals used for archivingheterozygous C57BL/6J

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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