STOCK Map3k8tm1.2Gkl/Flmg

Status

Available to order

EMMA IDEM:08371
International strain nameSTOCK Map3k8tm1.2Gkl/Flmg
Alternative nameB6-Map3k8/Flmg
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolMap3k8tm1.2Gkl,
Gene/Transgene symbolMap3k8

Information from provider

ProviderGeorge Kollias
Provider affiliationBSRC Al. Fleming
Genetic informationA three loxP targeting vector containing 5' and 3' homology arms with mouse Map3k8 (Tpl2) gene, a neomycin cassette flanked by loxP1 and loxP2 and exon 4 flanked by loxP2 and loxP3 was used for the generation of targeted mice through targeting of ES cells with vector and selection for homologous recombination. From mice bearing the homologous integration of targeting vector in the germ line both exon 4 and neomycin cassette were removed upon in vivo recombination by EIIa-Cre.
Phenotypic informationHomozygous:
No overt phenotype

Heterozygous:
No overt phenotype
References
  • Tpl2 regulates intestinal myofibroblast HGF release to suppress colitis-associated tumorigenesis.;Koliaraki Vasiliki, Roulis Manolis, Kollias George, ;2012;The Journal of clinical investigation;122;4231-42; 23064365
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreB.S.R.C. Alexander Fleming, Vari, Greece
Animals used for archivinghomozygous C57BL/6J

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal colon morphology / MGI
  • rectal hemorrhage / MGI
  • increased tumor growth/size / MGI
  • increased incidence of tumors by chemical induction / MGI
  • diarrhea / MGI
  • decreased tumor necrosis factor secretion / MGI
  • increased physiological sensitivity to xenobiotic / MGI
  • abnormal enterocyte proliferation / MGI
  • abnormal enterocyte apoptosis / MGI
  • increased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • decreased sensitivity to xenobiotic induced morbidity/mortality / MGI
  • increased susceptibility to weight loss / MGI
  • decreased colon length / MGI

Literature references

  • Tpl2 regulates intestinal myofibroblast HGF release to suppress colitis-associated tumorigenesis.;Koliaraki Vasiliki, Roulis Manolis, Kollias George, ;2012;The Journal of clinical investigation;122;4231-42; 23064365

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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