STOCK Ightm9.1Cog/Orl
| Status | Available to order |
| EMMA ID | EM:11091 |
| International strain name | STOCK Ightm9.1Cog/Orl |
| Alternative name | IRIS mice |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Ightm9.1Cog, |
| Gene/Transgene symbol | Igh |
Information from provider
| Provider | Yves Denizot |
| Provider affiliation | Immunology, UMR CNRS 7276 - University of Limoges |
| Genetic information | The IgH 3’ regulatory region (3’RR) controls class switch recombination (CSR) and somatic hypermutation (SHM) in B cells. The mouse 3’RR contains four enhancer elements with hs1,2 flanked by inverted repeated intervening sequences (IRIS) and the center of a 25-kb palindrome bounded by two hs3 enhancer inverted copies (hs3a and hs3b); hs4 lies downstream of the palindrome. To explore the role of the 3’RR palindromic architecture in the context of the endogenous locus, we analysed IRIS mice with a deconstructing palindrome by fully eliminating left-side IRIS while maintaining all four core enhancer elements. |
| Phenotypic information | Severe decrease of somatic hypermutation in VH gene. Partial decrease in class switch recombination and germline transcription. Number of B-cells not affected. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous 129S1/Sv-Oca2<+> Tyr<+> Kitl |
Literature references
- Deciphering the importance of the palindromic architecture of the immunoglobulin heavy-chain 3' regulatory region.;Saintamand Alexis, Vincent-Fabert Christelle, Garot Armand, Rouaud Pauline, Oruc Zeliha, Magnone Virginie, Cogné Michel, Denizot Yves, ;2016;Nature communications;7;10730; 26883548