Service Description:

The Mary Lyon Centre (MLC) at MRC Harwell offers a comprehensive genome engineering resource from design to model production and validation. It establishes and evolves new methods for the production of validated mouse models on controlled genetic backgrounds.

Additional Support:

• Project design and strategy optimisation based on years of experience in creating both simple and complex alleles.
• Supply/production of relevant reagents.
• Choice of a broad range of techniques, according to the required genetic alteration. These include classical gene targeting, genome editing using CRISPR/Cas9 in early embryos, CRISPR-aided targeting in embryonic stem cells, or additive transgenesis to generate the validated mouse.
• Allele types include tags, knock-ins, reporter and recombinase expressors, humanisation, inducible overexpression systems, large-scale deletions or duplication.
• Full validation of the genetic sequence of the mutant allele.
• Further functional validation of transgenes. This includes the option of mutant transcript quantification and recombinase activity profiling.
• They also offer the characterisation of copy number and point of insertion of transgenes obtained by additive transgenesis.