C57BL/6-Tg(Gt(ROSA)26Sor-ALPP)181Biat/Biat

Status	Available to order
EMMA ID	EM:10665
International strain name	C57BL/6-Tg(Gt(ROSA)26Sor-ALPP)181Biat/Biat
Alternative name	C57BL/6N-Tg(R26-ALPP)181Biat [Tg(ALPP)] LabName, Synonyms: ALPP donor strain (ALPP181), Tg(ALPP), li
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Gt(ROSA)26Sor-ALPP)181Biat
Gene/Transgene symbol	Tg(Gt(ROSA)26Sor-ALPP)181Biat

Information from provider

Provider	Reinhold Erben
Provider affiliation	Department 1 (Biomedical Sciences), University of Veterinary Medicine
Genetic information	This animal model is based on a dual transgenic system, consisting of a donor line (line 1) and a corresponding marker-tolerant recipient line (line 2) characterized by innate tolerance to the donor marker. ALPP (human placental alkaline phosphatase) was chosen as cell-tracking marker protein due to its ease of detection in several tissues, and because ALPP is known to be developmentally neutral in mice. The donor line Tg(ALPP) expresses heat stable wild-type ALPP in a ubiquitous fashion and serves as an unlimited source of genetically labeled cells and tissues.
Phenotypic information	Homozygous: No overt phenotype; histologically: ubiquitously positive staining (heat stable) of the human PLAP protein in transgenic animals. Heterozygous: No overt phenotype; histologically: ubiquitously positive staining (heat stable) of the human PLAP protein in transgenic animals.
Breeding history	Created in 2007 in C57BL/6, constantly crossed with C57BL/6N (20 generations minimum); breeding system: tg x C57BL/6; next generation: C57BL/6 x tg (male and female heredity alternating); keeping line 1 and 2 on an identical background is essential for this animal model! - background differences may cause immunoreactions in transplantation experiments independently from ALPP.
References	None available
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	no

Information from EMMA

Archiving centre	University of Veterinary Medicine, Vienna, Austria
Animals used for archiving	heterozygous C57BL/6N

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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