C57BL/6-Tg(Gt(ROSA)26Sor-ALPP*)192Biat/Biat
| Status | Available to order |
| EMMA ID | EM:10666 |
| International strain name | C57BL/6-Tg(Gt(ROSA)26Sor-ALPP*)192Biat/Biat |
| Alternative name | C57BL/6N-Tg(R26-ALPPm)192Biat [Tg(ALPPm)] ; LabNames: TG(ALPP |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Gt(ROSA)26Sor-ALPP*)192Biat |
| Gene/Transgene symbol | Tg(Gt(ROSA)26Sor-ALPP*)192Biat |
Information from provider
| Provider | Reinhold Erben |
| Provider affiliation | Department 1 (Biomedical Sciences), University of Veterinary Medicine, Vienna |
| Genetic information | To generate ALPP tolerant recipients (line 2), ALPP´s unique heat stability was used: changing glutamic acid 451 to glycine was sufficient to render the protein heat-labile. This heat-labile form of ALPP can easily be distinguished from wild-type ALPP by heat inactivation, similar to endogenous APs. These recipient animals show complete immuno-tolerance versus wild-type ALPP (versus cells/tissues, transplanted from the donor line), as this mutated form of ALPP is virtually identical to native ALPP. Mutations: Human Alkaline Phosphatase (hPLAP) and Gt(ROSA)26Sor promoter (R26) was amplified from transgenic Fischer 344 rats ubiquitously expressing hPLAP (Kisseberth et al, 1999, Developmental Biology 214, 128 - 138). A single point mutation (A to G) was introduced via splice overlap extension resulting in an amino acid substitution at position 429 (glutamic acid to glycine); effect: loss of heat stability of the gene product. Teansgenic animals were created by pronuclear injection of mouse zygotes and random integration of the transgene. Phenotype: ubiquitous expression (low level) of the mutated marker protein hPLAP (heat labile); size of transgene: 5142 bp. |
| Phenotypic information | Homozygous:No overt phenotype; histologically: ubiquitously positive staining of the human PLAP protein in transgenic animals, gone after heat inactivation (like mouse AP)Heterozygous:No overt phenotype; histologically: ubiquitously positive staining of the human PLAP protein in transgenic animals, gone after heat inactivation (like mouse AP) |
| Breeding history | Created in 2007 on C57BL/6, constantly crossed with C57BL/6N (20 generations minimum); breeding system: tg x C57BL/6; next generation: C57BL/6 x tg (male and female heredity alternating) |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous C57BL/6N |