B6.Cg-Sdhbtm1Dost/H
| Status | Available to order |
| EMMA ID | EM:11640 |
| International strain name | B6.Cg-Sdhbtm1Dost/H |
| Alternative name | Sdhb-tm1Dost |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Sdhbtm1Dost, |
| Gene/Transgene symbol | Sdhb |
Information from provider
| Provider | Douglas Strathdee |
| Provider affiliation | CRUK Beatson Institute |
| Genetic information | A hygromycin resistance cassette with a 3' loxP site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. |
| Phenotypic information | Homozygous:Homozygous floxed mice are viable, fertile and have a normal lifespan. However after cre recombination homozygous knockouts may be infertile.Heterozygous:Heterozygous floxed mice are viable, fertile and have a normal lifespan. |
| Breeding history | Mice were crossed to a strain containing the Tg(ACTFLPe)9205Dym transgene. Expression of Flp recombinase was used to remove the hygromycin resistance gene. Subsequent backcrossing to C57BL/6J allowed the segregation of the flox allele. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | heterozygous C57BL/6J |
| Breeding at archiving centre | Backcrossed onto C57BL/6J for 10 generations |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Carney-Stratakis syndrome / Orphanet_97286
- Hereditary pheochromocytoma-paraganglioma / Orphanet_29072
- Isolated succinate-CoQ reductase deficiency / Orphanet_3208
- Cowden syndrome / Orphanet_201
Literature references
- Pyruvate carboxylation enables growth of SDH-deficient cells by supporting aspartate biosynthesis.;Cardaci Simone, Zheng Liang, MacKay Gillian, van den Broek Niels J F, MacKenzie Elaine D, Nixon Colin, Stevenson David, Tumanov Sergey, Bulusu Vinay, Kamphorst Jurre J, Vazquez Alexei, Fleming Stewart, Schiavi Francesca, Kalna Gabriela, Blyth Karen, Strathdee Douglas, Gottlieb Eyal, ;2015;Nature cell biology;17;1317-26; 26302408