STOCK Loxl2tm1.2Acan/Cnbc
Status | Available to order |
EMMA ID | EM:12882 |
International strain name | STOCK Loxl2tm1.2Acan/Cnbc |
Alternative name | CFB |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Loxl2tm1.2Acan, |
Gene/Transgene symbol | Loxl2 |
Information from provider
Provider | Amparo Cano |
Provider affiliation | Biochemistry, Autonoma University (Medicine Faculty) |
Additional owner | Francisco Portillo and Alberto Martin, Universidad Autónoma de Madrid, Spain. |
Genetic information | Constitutive Loxl2 knock-out. A conditional knock-out mutation of Loxl2 based on cre recombinase/loxP system was introduced by homologous recombination in the endogenous locus of Loxl2. |
Phenotypic information | Homozygous:Partial perinatal lethality due to cardiac and liver defects. Knock-out mice develop a lower number of skin tumors after being subjected to DMBA/TPA (dimethylbenz-anthracene and 12-O-tetradecanoyl phorbol-13-acetate) treatment.Heterozygous:Completely normal |
Breeding history | ES cell clones positive for the mutation were microinjected/aggregated into receptive blastocysts/morulas and chimeras were obtained. These animals were crossed with cre recombinase-positive mice in order to check germline transmission and, at the same time, obtain animals harbouring a constitutive knock-out allele. The resultant heterozygous animals were intercrossed and knock-out animals were obtained. |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Animals used for archiving | homozygous mixed 129S/SvEv and C57BL/6 and FVB, N5 C57BL/6, wild-type C57BL/6J |
Stage of embryos | 2-cell |
Literature references
- Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression.;Martin Alberto, Salvador Fernando, Moreno-Bueno Gema, Floristán Alfredo, Ruiz-Herguido Cristina, Cuevas Eva P, Morales Saleta, Santos Vanesa, Csiszar Katalin, Dubus Pierre, Haigh Jody J, Bigas Anna, Portillo Francisco, Cano Amparo, ;2015;The EMBO journal;34;1090-109; 25759215
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