- increased inflammatory response / MGI
- male infertility / MGI
- abnormal seminiferous tubule morphology / MGI
- abnormal epididymis morphology / MGI
- abnormal keratinocyte differentiation / MGI
- epididymal inflammation / MGI
- increased incidence of tumors by chemical induction / MGI
- abnormal epididymis epithelium morphology / MGI
- decreased tumor latency / MGI
- altered tumor pathology / MGI
- testis degeneration / MGI
- epididymis hypertrophy / MGI
- spermatocele / MGI
- epididymis fibrosis / MGI
- increased epididymal cell proliferation / MGI
- increased epididymal epithelium cell proliferation / MGI
STOCK Gt(ROSA)26Sortm1.1(Loxl2)Acan/Cncb
Status | Available to order |
EMMA ID | EM:12917 |
International strain name | STOCK Gt(ROSA)26Sortm1.1(Loxl2)Acan/Cncb |
Alternative name | ODB |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Gt(ROSA)26Sortm1.1(Loxl2)Acan, |
Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
Provider | Amparo Cano |
Provider affiliation | Biochemistry, Autonoma University (Medicine Faculty) |
Additional owner | Francisco Portillo and Alberto Martin, Universidad Autónoma de Madrid, Spain. |
Genetic information | Loxl2 constitutive knock-in. Loxl2 cDNA followed by a GFP reporter gene and an upstream PGK-neo cassette flanked by two LoxP sites were introduced by homologous recombination in the endogenous Gt(ROSA)26Sor locus. |
Phenotypic information | Homozygous:Homozygous males were sterile in almost 100% of the cases, in the studied genetic background, due to epididymal and testicular dysfunction.Heterozygous:Normal |
Breeding history | ES clones positive for the mutation were microinjected/aggregated into receptive blastocysts/morula and chimeras were obtained. These animals were crossed with cre recombinase-positive mice in order to check germline transmission and, at the same time, obtain animals constitutively expressing extra amounts of Loxl2. The resultant heterozygous animals were intercrossed and homozygous animals harboring two Loxl2 over-expression alleles were obtained. |
References |
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Homozygous fertile | females only |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
Animals used for archiving | heterozygous mixed, wild-type C57BL/6J |
Stage of embryos | 2-cell |
Disease and phenotype information
MGI phenotypes (allele matching)
Literature references
- Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression.;Martin Alberto, Salvador Fernando, Moreno-Bueno Gema, Floristán Alfredo, Ruiz-Herguido Cristina, Cuevas Eva P, Morales Saleta, Santos Vanesa, Csiszar Katalin, Dubus Pierre, Haigh Jody J, Bigas Anna, Portillo Francisco, Cano Amparo, ;2015;The EMBO journal;34;1090-109; 25759215
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