MGI phenotypes (allele matching)
- no phenotypic analysis / MGI
STOCK Gt(ROSA)26Sortm1(Loxl2)Acan/Cnbc
| Status | Available to order |
| EMMA ID | EM:13006 |
| International strain name | STOCK Gt(ROSA)26Sortm1(Loxl2)Acan/Cnbc |
| Alternative name | FC |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Gt(ROSA)26Sortm1(Loxl2)Acan, |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | Amparo Cano |
| Provider affiliation | Biochemistry, Autonoma University (Medicine Faculty) |
| Additional owner | Francisco Portillo and Alberto Martin, Universidad Autónoma de Madrid, Spain. |
| Genetic information | Loxl2 conditional knock-in. Loxl2 cDNA followed by a GFP reporter gene and an upstream PGK-neo cassette flanked by two loxP sites were introduced by homologous recombination in the endogenous Gt(ROSA)26Sor locus. |
| Phenotypic information | Homozygous:No remarkable phenotype. Animals are similar to wild-type.Heterozygous:No remarkable phenotype. Animals are similar to wild-type. |
| Breeding history | ES cells clones positive for the mutation were microinjected/aggregated into receptive blastocysts/morulas and chimeras were obtained. These animals were crossed with wild-type mice in order to check germline transmission. The resultant heterozygous animals for the conditional knock-in mutation of Loxl2 were intercrossed and homozygous animals were obtained. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous mixed, homozygous mixed |
| Stage of embryos | 2-cell |
Disease and phenotype information
Literature references
- Lysyl oxidase-like 2 represses Notch1 expression in the skin to promote squamous cell carcinoma progression.;Martin Alberto, Salvador Fernando, Moreno-Bueno Gema, Floristán Alfredo, Ruiz-Herguido Cristina, Cuevas Eva P, Morales Saleta, Santos Vanesa, Csiszar Katalin, Dubus Pierre, Haigh Jody J, Bigas Anna, Portillo Francisco, Cano Amparo, ;2015;The EMBO journal;34;1090-109; 25759215
- Lysyl Oxidase-like Protein LOXL2 Promotes Lung Metastasis of Breast Cancer.;Salvador Fernando, Martin Alberto, López-Menéndez Celia, Moreno-Bueno Gema, Santos Vanesa, Vázquez-Naharro Alberto, Santamaria Patricia G, Morales Saleta, Dubus Pierre R, Muinelo-Romay Laura, López-López Rafael, Tung Jason C, Weaver Valerie M, Portillo Francisco, Cano Amparo, ;2017;Cancer research;77;5846-5859; 28720577