B6-Tnfrsf1btm1Gkl/Flmg
| Status | Available to order |
| EMMA ID | EM:13086 |
| International strain name | B6-Tnfrsf1btm1Gkl/Flmg |
| Alternative name | B6-Tnfrsf1b |
| Strain type | Targeted Mutant Strains |
| Allele/Transgene symbol | Tnfrsf1b |
| Gene/Transgene symbol | Tnfrsf1b |
Information from provider
| Provider | George Kollias |
| Provider affiliation | BSRC "Alexander Fleming" |
| Genetic information | The conditional Tnfrsf1bf/f mouse strain, was generated using standard gene-targeting techniques. A targeting vector was generated to induce homologous recombination in ES cells, with exon 2 flanked by loxP sites, which -upon recombination- would result in a frame shift mutation. The vector was introduced in Bruce 4 ES cells, and clones, having successfully recombined, were used for the generation of chimaeras. Chimeric mice were crossed to C57BL/6J mice, and offspring with germline transmission of the mutated allele were used for expansion. |
| Phenotypic information | Homozygous:No overt phenotypeHeterozygous:No overt phenotype |
| Breeding history | The plasmid was first introduced in in Bruce 4 ES cells and clones were used to generate chimeras. Succesful germline trasmission mice were back-crossed in C57BL/6 genetic background mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | B.S.R.C. Alexander Fleming, Vari, Greece |
| Animals used for archiving | homozygous C57BL/6J |
Literature references
- Mesenchymal TNFR2 promotes the development of polyarthritis and comorbid heart valve stenosis.;Sakkou Maria, Chouvardas Panagiotis, Ntari Lydia, Prados Alejandro, Moreth Kristin, Fuchs Helmut, Gailus-Durner Valerie, Hrabe de Angelis Martin, Denis Maria C, Karagianni Niki, Kollias George, ;2018;JCI insight;3;; 29618659