B6;129-Lamtor2tm1.1Lah/Biat
| Status | Available to order |
| EMMA ID | EM:13088 |
| International strain name | B6;129-Lamtor2tm1.1Lah/Biat |
| Alternative name | LAMTOR2 flox/- |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Lamtor2tm1.1Lah, |
| Gene/Transgene symbol | Lamtor2 |
Information from provider
| Provider | Lukas A. Huber |
| Provider affiliation | Biocenter, Cell Biology, Medical University Innsbruck |
| Additional owner | Tarek Moustafa, Division of Gastroenterology and Hepatology, Medical University Graz, Austria |
| Genetic information | Exons 1-4 of Lamtor2 (LT2) are flanked by loxP sites. HM-1 embryonic stem cells were transfected with the targeting vector. Two clones with one targeted Lamtor2 allele were selected and injected into C57BL/6 blastocysts. The generated chimeric mice were crossed with C57BL/6 mice to obtain germ-line transmission of the targeted Lamtor2 allele, LT2targ/+. LT2targ/targ mice were crossed with hACTB::Flpe mice to obtain mice with floxed Lamtor2 alleles (LT2flox/+) as Flpe mediated the deletion of the frt flanked PGK-Neo cassette. Crossing the LT2targ/targ mice with MORE mice (Tal-lquist and Soriano, 2000) generated LT2-/+ mice harbouring a heterozygous p14 gene deletion. These mice were further on crossed with LT2flox/flox mice to obtain the Lamtor2 flox/- mice. |
| Phenotypic information | Homozygous:A homozygous deletion of Lamtor2 is embryonic lethal. The homozygous floxed alleles of Lamtor2 show no phenotype without Cre deletion.Heterozygous:The Lamtor2 flox/delta mice show no phenotype without Cre deletion. |
| Breeding history | The strain has been inbred on C57BL/6 mouse strain for at least 10 sib-matings. |
| References |
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| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous C57BL/6J |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Primary immunodeficiency syndrome due to LAMTOR2 deficiency / Orphanet_90023
Literature references
- p14-MP1-MEK1 signaling regulates endosomal traffic and cellular proliferation during tissue homeostasis.;Teis David, Taub Nicole, Kurzbauer Robert, Hilber Diana, de Araujo Mariana E, Erlacher Miriam, Offterdinger Martin, Villunger Andreas, Geley Stephan, Bohn Georg, Klein Christoph, Hess Michael W, Huber Lukas A, ;2006;The Journal of cell biology;175;861-8; 17178906