B6.129(Cg)-Sirt1tm1.1Auw/Orl
| Status | Available to order |
| EMMA ID | EM:13154 |
| International strain name | B6.129(Cg)-Sirt1tm1.1Auw/Orl |
| Alternative name | Sirt1tm1.1Auw |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Sirt1 |
| Gene/Transgene symbol | Sirt1 |
Information from provider
| Provider | Thibaud Clerc |
| Provider affiliation | SV IBI LISP, EPFL |
| Genetic information | Exons 5, 6, and 7 were flanked by loxP sites and an FRT flanked selection cassette was inserted. Properly targeted mice were bred to mice expressing flp recombinase in order to excise the selection cassette. Location: Chr10:63319005-63339035 bp, - strand Genetic Position: Chr10, 32.57 cM |
| Phenotypic information | Homozygous:Mice that are homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.Heterozygous:Mice that are heterozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. |
| Breeding history | ES cells were injected in 129/Sv mouse. Properly targeted mice were bred to mice expressing flp recombinase in order to excise the selection cassette. Then 10 backcrosses were done with C57BL/6J mice. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | homozygous C57BL/6 |
Literature references
- The NAD(+)/Sirtuin Pathway Modulates Longevity through Activation of Mitochondrial UPR and FOXO Signaling.;Mouchiroud Laurent, Houtkooper Riekelt H, Moullan Norman, Katsyuba Elena, Ryu Dongryeol, Cantó Carles, Mottis Adrienne, Jo Young-Suk, Viswanathan Mohan, Schoonjans Kristina, Guarente Leonard, Auwerx Johan, ;2013;Cell;154;430-41; 23870130