B6.129S2-Sulf2^tm1Clrm/Ieg

Status	Available to order
EMMA ID	EM:13163
Citation information	RRID:IMSR_EM:13163 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6.129S2-Sulf2^tm1Clrm/Ieg
Alternative name	Sulf2tm1Clrm
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Sulf2^tm1Clrm
Gene/Transgene symbol	Sulf2

Information from provider

Provider	Torben Lübke
Provider affiliation	Chemistry - Biochemistry, Bielefeld University
Genetic information	Targeted mutation (null/knockout) in the heparan sulfate 6-O-endosulfatase (Sulf2), sulfatase 2, gene. Mutation details: a neomycin resistance gene was inserted into exon 1. Northern blot confirmed absence of transcript in mutants. PMID:16901266
Phenotypic information	Homozygous: See also http://www.informatics.jax.org/allele/allgenoviews/MGI:3699621 premature death ( J:116527 ) decreased body weight ( J:116527 ) abnormal brain morphology ( J:116527 ) Heterozygous: No obvious phenotype
Breeding history	Backcrossed for at least 10 generations into C57BL/6JRccHsd
References	Roles of heparan sulfate sulfation in dentinogenesis.;Hayano Satoru, Kurosaka Hiroshi, Yanagita Takeshi, Kalus Ina, Milz Fabian, Ishihara Yoshihito, Islam Md Nurul, Kawanabe Noriaki, Saito Masahiro, Kamioka Hiroshi, Adachi Taiji, Dierks Thomas, Yamashiro Takashi, ;2012;The Journal of biological chemistry;287;12217-29; 22351753 Sulf1 and Sulf2 Differentially Modulate Heparan Sulfate Proteoglycan Sulfation during Postnatal Cerebellum Development: Evidence for Neuroprotective and Neurite Outgrowth Promoting Functions.;Kalus Ina, Rohn Susanne, Puvirajesinghe Tania M, Guimond Scott E, Eyckerman-Kölln Pieter J, Ten Dam Gerdy, van Kuppevelt Toin H, Turnbull Jeremy E, Dierks Thomas, ;2015;PloS one;10;e0139853; 26448642 Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development.;Ratzka Andreas, Kalus Ina, Moser Markus, Dierks Thomas, Mundlos Stefan, Vortkamp Andrea, ;2008;Developmental dynamics : an official publication of the American Association of Anatomists;237;339-53; 18213582 Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity.;Lamanna William C, Baldwin Rebecca J, Padva Michael, Kalus Ina, Ten Dam Gerdy, van Kuppevelt Toin H, Gallagher John T, von Figura Kurt, Dierks Thomas, Merry Catherine L R, ;2006;The Biochemical journal;400;63-73; 16901266 Heparan Sulfate-Editing Extracellular Sulfatases Enhance VEGF Bioavailability for Ischemic Heart Repair.;Korf-Klingebiel Mortimer, Reboll Marc R, Grote Karsten, Schleiner Hauke, Wang Yong, Wu Xuekun, Klede Stefanie, Mikhed Yuliya, Bauersachs Johann, Klintschar Michael, Rudat Carsten, Kispert Andreas, Niessen Hans W, Lübke Torben, Dierks Thomas, Wollert Kai C, ;2019;Circulation research;125;787-801; 31434553 Differential involvement of the extracellular 6-O-endosulfatases Sulf1 and Sulf2 in brain development and neuronal and behavioural plasticity.;Kalus Ina, Salmen Benedikt, Viebahn Christoph, von Figura Kurt, Schmitz Dietmar, D'Hooge Rudi, Dierks Thomas, ;2009;Journal of cellular and molecular medicine;13;4505-21; 20394677 The SULFs, extracellular sulfatases for heparan sulfate, promote the migration of corneal epithelial cells during wound repair.;Maltseva Inna, Chan Matilda, Kalus Ina, Dierks Thomas, Rosen Steven D, ;2013;PloS one;8;e69642; 23950901
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	no
Immunocompromised	not known

Information from EMMA

Archiving centre	Helmholtz Zentrum Muenchen - German Research Center for Environmental Health (GmbH), Oberschleißheim, Germany
Animals used for archiving	homozygous C57BL/6 males

Literature references

Roles of heparan sulfate sulfation in dentinogenesis.;Hayano Satoru, Kurosaka Hiroshi, Yanagita Takeshi, Kalus Ina, Milz Fabian, Ishihara Yoshihito, Islam Md Nurul, Kawanabe Noriaki, Saito Masahiro, Kamioka Hiroshi, Adachi Taiji, Dierks Thomas, Yamashiro Takashi, ;2012;The Journal of biological chemistry;287;12217-29; 22351753
Sulf1 and Sulf2 Differentially Modulate Heparan Sulfate Proteoglycan Sulfation during Postnatal Cerebellum Development: Evidence for Neuroprotective and Neurite Outgrowth Promoting Functions.;Kalus Ina, Rohn Susanne, Puvirajesinghe Tania M, Guimond Scott E, Eyckerman-Kölln Pieter J, Ten Dam Gerdy, van Kuppevelt Toin H, Turnbull Jeremy E, Dierks Thomas, ;2015;PloS one;10;e0139853; 26448642
Redundant function of the heparan sulfate 6-O-endosulfatases Sulf1 and Sulf2 during skeletal development.;Ratzka Andreas, Kalus Ina, Moser Markus, Dierks Thomas, Mundlos Stefan, Vortkamp Andrea, ;2008;Developmental dynamics : an official publication of the American Association of Anatomists;237;339-53; 18213582
Heparan sulfate 6-O-endosulfatases: discrete in vivo activities and functional co-operativity.;Lamanna William C, Baldwin Rebecca J, Padva Michael, Kalus Ina, Ten Dam Gerdy, van Kuppevelt Toin H, Gallagher John T, von Figura Kurt, Dierks Thomas, Merry Catherine L R, ;2006;The Biochemical journal;400;63-73; 16901266
Heparan Sulfate-Editing Extracellular Sulfatases Enhance VEGF Bioavailability for Ischemic Heart Repair.;Korf-Klingebiel Mortimer, Reboll Marc R, Grote Karsten, Schleiner Hauke, Wang Yong, Wu Xuekun, Klede Stefanie, Mikhed Yuliya, Bauersachs Johann, Klintschar Michael, Rudat Carsten, Kispert Andreas, Niessen Hans W, Lübke Torben, Dierks Thomas, Wollert Kai C, ;2019;Circulation research;125;787-801; 31434553
Differential involvement of the extracellular 6-O-endosulfatases Sulf1 and Sulf2 in brain development and neuronal and behavioural plasticity.;Kalus Ina, Salmen Benedikt, Viebahn Christoph, von Figura Kurt, Schmitz Dietmar, D'Hooge Rudi, Dierks Thomas, ;2009;Journal of cellular and molecular medicine;13;4505-21; 20394677
The SULFs, extracellular sulfatases for heparan sulfate, promote the migration of corneal epithelial cells during wound repair.;Maltseva Inna, Chan Matilda, Kalus Ina, Dierks Thomas, Rosen Steven D, ;2013;PloS one;8;e69642; 23950901

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

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B6.129S2-Sulf2tm1Clrm/Ieg