STOCK Gt(ROSA)26Sortm1(GSDMB_i2,-EGFP)Gmbu/Cnbc
| Status | Available to order |
| EMMA ID | EM:13205 |
| International strain name | STOCK Gt(ROSA)26Sortm1(GSDMB_i2,-EGFP)Gmbu/Cnbc |
| Alternative name | R26-STOP-GB2 |
| Strain type | Targeted Mutant Strains : Conditional mutation |
| Allele/Transgene symbol | Gt(ROSA)26Sortm1(GSDMB_i2, -EGFP)Gmbu |
| Gene/Transgene symbol | Gt(ROSA)26Sor |
Information from provider
| Provider | GEMA MORENO-BUENO |
| Provider affiliation | BIOCHEMISTRY, AUTONOMOUS UNIVERSITY OF MADRID (UAM) |
| Genetic information | Conditional knock-in (KI) model harbouring the human GSDMB isoform 2 transcript (NM_018530.2) within the ROSA26 (R26) endogenous locus. Using the gene-targeting technology described before (Nyabi et al. Nucleic Acids Res. 2009) we inserted into the R26 locus a construct containing a loxP-flanked PGK-neomycin-STOP cassette followed by the human GSDMB isoform 2 cDNA (GSDMB2) fused with the HA-tag sequence. The construct also contains an IRES-sequence followed by the Green Fluorescent Protein (GFP) gene reporter, which helps in the identification of the Knock-in (KI) animals. Expression of the GSDMB2-HA and GFP transgenes is prevented by the presence of the PGK-neomycin-STOP cassette. After crossing this strain with cre-expressing models, the STOP cassette is excised and the expression of the GSDMB2-HA and GFP genes regulated by the endogenous R26 promoter. |
| Phenotypic information | Homozygous:This is a conditional knock-in model. Expression of the transgenes is prevented by the upstream STOP cassette. Therefore, it shows no phenotypic effect. It requires crossing with a cre-expressing strain to activate expression of the transgenes.Heterozygous:This is a conditional knock-in model. Expression of the transgenes is prevented by the upstream STOP cassette. Therefore, it shows no phenotypic effect. It requires crossing with a cre-expressing strain to activate expression of the transgenes. |
| Breeding history | The ES cells (C57BL/6 x 129 background) were used for aggregation with CD1 embryos. The chimeric animals were crossed with FVB/NCrl wild-type mice to verify the correct transmission of the transgenes. The R26-STOP-GB2 strain was then backcrossed with the FVB/NCrl strain. Next, homozygous GSDMB2-HA/GFP animals were crossed (sibling matings) for 5 generations before being backcrossed again with the FVB/NCrl strain. Currently, homozygous GSDMB2-HA/GFP animals have been crossed (sibling matings) for additional 6 generations. Thus, the model has mixed background that is enriched in the FVB genetic makeup (total 3 backcrosses with this strain). |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous FVB/N, homozygous FVB/N |
Literature references
- Gasdermin-B Pro-Tumor Function in Novel Knock-in Mouse Models Depends on the in vivo Biological Context.;Sarrio David, Rojo-Sebastián Alejandro, Teijo Ana, Pérez-López María, Díaz-Martín Eva, Martínez Lidia, Morales Saleta, García-Sanz Pablo, Palacios José, Moreno-Bueno Gema, ;2022;Frontiers in cell and developmental biology;10;813929; 35281099