- abnormal ovarian follicle morphology / MGI
- absent corpus luteum / MGI
- small testis / MGI
- small seminiferous tubules / MGI
- abnormal spermatogenesis / MGI
- reduced female fertility / MGI
- decreased litter size / MGI
- decreased mature ovarian follicle number / MGI
- oligozoospermia / MGI
- maternal effect / MGI
- ovary hemorrhage / MGI
- decreased testis weight / MGI
- decreased epididymis weight / MGI
- abnormal male meiosis / MGI
- decreased oocyte number / MGI
- abnormal female germ cell morphology / MGI
- abnormal male germ cell apoptosis / MGI
- small hippocampus / MGI
- enlarged lateral ventricles / MGI
- enlarged third ventricle / MGI
- ovarian follicular cyst / MGI
- abnormal synaptonemal complex / MGI
B6.129S2-Hsf2tm1Mmr/Orl
Status | Available to order |
EMMA ID | EM:01636 |
International strain name | B6.129S2-Hsf2tm1Mmr/Orl |
Alternative name | Hsf2KO |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Hsf2tm1Mmr, |
Gene/Transgene symbol | Hsf2 |
Information from provider
Provider | Valerie Mezger |
Provider affiliation | Ecole Normale Superieure |
Genetic information | The Hsf2 gene was disrupted by insertion of the beta-geo gene at the 5' of exon 5 (oligomerization domain sequence), using a promoterless construct. |
Phenotypic information | Hsf2 knock-out mice are not sterile but display meiotic defects due to the synaptonemal complex, in both genders. Males show a 60% reduction in sperm count but are fertile. Females are hypofertile and it is better to maintain the strain by breeding heterozygous individuals. Hsf2 knock-out mice also display brain abnormalities characterized by the enlargement of ventricles, reduction in hippocampus size and cortical abnormalities. |
Breeding history | Recombinant 129 ES cells were injected into C57BL/6 blastocysts. One female transmitted the recombinant allele to its progeny. Mice were backcrossed for 8 generations to the C57BL/6 background. We cross only heterozygous individuals because of the meiotic phenotype. There is a possibility of a mix between the B6J and B6N background. |
References |
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Information from EMMA
Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
Disease and phenotype information
MGI phenotypes (allele matching)
Literature references
- Genomic structure and chromosomal localization of the mouse Hsf2 gene and promoter sequences.;Manuel M, Sage J, Mattéi M G, Morange M, Mezger V, ;1999;Gene;232;115-24; 10333528
- Brain abnormalities, defective meiotic chromosome synapsis and female subfertility in HSF2 null mice.;Kallio Marko, Chang Yunhua, Manuel Martine, Alastalo Tero-Pekka, Rallu Murielle, Gitton Yorick, Pirkkala Lila, Loones Marie-Thérèse, Paslaru Liliana, Larney Severine, Hiard Sophie, Morange Michel, Sistonen Lea, Mezger Valérie, ;2002;The EMBO journal;21;2591-601; 12032072
- Phenotypic characterization of mouse embryonic fibroblasts lacking heat shock factor 2.;Paslaru Liliana, Morange M, Mezger Valérie, ;2003;Journal of cellular and molecular medicine;7;425-35; 14754511
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