B6.129S2-Hsf2tm1Mmr/Orl

Status

Available to order

EMMA IDEM:01636
International strain nameB6.129S2-Hsf2tm1Mmr/Orl
Alternative nameHsf2KO
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolHsf2tm1Mmr,
Gene/Transgene symbolHsf2

Information from provider

ProviderValerie Mezger
Provider affiliationEcole Normale Superieure
Genetic informationThe Hsf2 gene was disrupted by insertion of the beta-geo gene at the 5' of exon 5 (oligomerization domain sequence), using a promoterless construct.
Phenotypic informationHsf2 knock-out mice are not sterile but display meiotic defects due to the synaptonemal complex, in both genders. Males show a 60% reduction in sperm count but are fertile. Females are hypofertile and it is better to maintain the strain by breeding heterozygous individuals. Hsf2 knock-out mice also display brain abnormalities characterized by the enlargement of ventricles, reduction in hippocampus size and cortical abnormalities.
Breeding historyRecombinant 129 ES cells were injected into C57BL/6 blastocysts. One female transmitted the recombinant allele to its progeny. Mice were backcrossed for 8 generations to the C57BL/6 background. We cross only heterozygous individuals because of the meiotic phenotype. There is a possibility of a mix between the B6J and B6N background.
References
  • Genomic structure and chromosomal localization of the mouse Hsf2 gene and promoter sequences.;Manuel M, Sage J, Mattéi M G, Morange M, Mezger V, ;1999;Gene;232;115-24; 10333528
  • Brain abnormalities, defective meiotic chromosome synapsis and female subfertility in HSF2 null mice.;Kallio Marko, Chang Yunhua, Manuel Martine, Alastalo Tero-Pekka, Rallu Murielle, Gitton Yorick, Pirkkala Lila, Loones Marie-Thérèse, Paslaru Liliana, Larney Severine, Hiard Sophie, Morange Michel, Sistonen Lea, Mezger Valérie, ;2002;The EMBO journal;21;2591-601; 12032072
  • Phenotypic characterization of mouse embryonic fibroblasts lacking heat shock factor 2.;Paslaru Liliana, Morange M, Mezger Valérie, ;2003;Journal of cellular and molecular medicine;7;425-35; 14754511

Information from EMMA

Archiving centreInstitut de Transgenose, INTRAGENE, Orléans, France

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal ovarian follicle morphology / MGI
  • absent corpus luteum / MGI
  • small testis / MGI
  • small seminiferous tubules / MGI
  • abnormal spermatogenesis / MGI
  • reduced female fertility / MGI
  • decreased litter size / MGI
  • decreased mature ovarian follicle number / MGI
  • oligozoospermia / MGI
  • maternal effect / MGI
  • ovary hemorrhage / MGI
  • decreased testis weight / MGI
  • decreased epididymis weight / MGI
  • abnormal male meiosis / MGI
  • decreased oocyte number / MGI
  • abnormal female germ cell morphology / MGI
  • abnormal male germ cell apoptosis / MGI
  • small hippocampus / MGI
  • enlarged lateral ventricles / MGI
  • enlarged third ventricle / MGI
  • ovarian follicular cyst / MGI
  • abnormal synaptonemal complex / MGI

Literature references

  • Genomic structure and chromosomal localization of the mouse Hsf2 gene and promoter sequences.;Manuel M, Sage J, Mattéi M G, Morange M, Mezger V, ;1999;Gene;232;115-24; 10333528
  • Brain abnormalities, defective meiotic chromosome synapsis and female subfertility in HSF2 null mice.;Kallio Marko, Chang Yunhua, Manuel Martine, Alastalo Tero-Pekka, Rallu Murielle, Gitton Yorick, Pirkkala Lila, Loones Marie-Thérèse, Paslaru Liliana, Larney Severine, Hiard Sophie, Morange Michel, Sistonen Lea, Mezger Valérie, ;2002;The EMBO journal;21;2591-601; 12032072
  • Phenotypic characterization of mouse embryonic fibroblasts lacking heat shock factor 2.;Paslaru Liliana, Morange M, Mezger Valérie, ;2003;Journal of cellular and molecular medicine;7;425-35; 14754511

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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