B6;CBA-Tg(Igf2/LacZ,H19)YZ17Aco/AcoH

Status	Available to order
EMMA ID	EM:02379
Citation information	RRID:IMSR_EM:02379 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information.
International strain name	B6;CBA-Tg(Igf2/LacZ,H19)YZ17Aco/AcoH
Alternative name	YZ17
Strain type	Transgenic Strains
Allele/Transgene symbol	Tg(Igf2/LacZ,H19)YZ17Aco
Gene/Transgene symbol	Tg(Igf2/LacZ,H19)YZ17Aco

Information from provider

Provider	Justin Ainscough
Provider affiliation	Central Biomedical Services, University of Leeds
Genetic information	The YZ17 line (two copy transgene) contains an additional 130 kb genomic region from mouse distal chromosome 7 spanning from Igf2 to ~40 kb downstream of H19. Igf2 has been inactivated by insertional mutagenesis resulting in an Igf2-LacZ gene fusion. A 1kb conserved intergenic region is flanked with LoxP sites. LacZ activity shows spatial expression of Igf2 in a subset of tissues. The imprinted H19 gene is only expressed when transmitted through the maternal germline. After paternal transmission the gene is repressed. The Igf2-LacZ gene shows appropriate reciprocal imprinting (paternal allele specific expression, maternal repression). Deletion of the intergenic region with Cre recombinase results in partial (mesoderm specific) reactivation of Igf2-LacZ expression following maternal transmission.
Phenotypic information	The line has not been kept homozygous due to reduced fertility.
Breeding history	Brother sister matings with sporadic backcrossing to C57BL/6.
References	A skeletal muscle-specific mouse Igf2 repressor lies 40 kb downstream of the gene.;Ainscough J F, John R M, Barton S C, Surani M A, ;2000;Development (Cambridge, England);127;3923-30; 10952890

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom

Literature references

A skeletal muscle-specific mouse Igf2 repressor lies 40 kb downstream of the gene.;Ainscough J F, John R M, Barton S C, Surani M A, ;2000;Development (Cambridge, England);127;3923-30; 10952890

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