B6;CBA-Tg(Igf2/LacZ,H19)YZ17Aco/AcoH
Status | Available to order |
EMMA ID | EM:02379 |
International strain name | B6;CBA-Tg(Igf2/LacZ,H19)YZ17Aco/AcoH |
Alternative name | YZ17 |
Strain type | Transgenic Strains |
Allele/Transgene symbol | Tg(Igf2/LacZ, H19)YZ17Aco |
Gene/Transgene symbol | Tg(Igf2/LacZ,H19)YZ17Aco |
Information from provider
Provider | Justin Ainscough |
Provider affiliation | Central Biomedical Services, University of Leeds |
Genetic information | The YZ17 line (two copy transgene) contains an additional 130 kb genomic region from mouse distal chromosome 7 spanning from Igf2 to ~40 kb downstream of H19. Igf2 has been inactivated by insertional mutagenesis resulting in an Igf2-LacZ gene fusion. A 1kb conserved intergenic region is flanked with LoxP sites. LacZ activity shows spatial expression of Igf2 in a subset of tissues. The imprinted H19 gene is only expressed when transmitted through the maternal germline. After paternal transmission the gene is repressed. The Igf2-LacZ gene shows appropriate reciprocal imprinting (paternal allele specific expression, maternal repression). Deletion of the intergenic region with Cre recombinase results in partial (mesoderm specific) reactivation of Igf2-LacZ expression following maternal transmission. |
Phenotypic information | The line has not been kept homozygous due to reduced fertility. |
Breeding history | Brother sister matings with sporadic backcrossing to C57BL/6. |
References |
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Information from EMMA
Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Literature references
- A skeletal muscle-specific mouse Igf2 repressor lies 40 kb downstream of the gene.;Ainscough J F, John R M, Barton S C, Surani M A, ;2000;Development (Cambridge, England);127;3923-30; 10952890
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