MGI phenotypes (allele matching)
B6.129P2-Sp1tm1Phi/Cnrm
| Status | Available to order |
| EMMA ID | EM:02430 |
| International strain name | B6.129P2-Sp1tm1Phi/Cnrm |
| Alternative name | Sp1 knockout |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Sp1tm1Phi, |
| Gene/Transgene symbol | Sp1 |
Information from provider
| Provider | Sjaak Philipsen |
| Provider affiliation | Genetics, Erasmus MC |
| Genetic information | A 129 mouse cosmid genomic DNA library was screened with a probe spanning nucleotides 1820-2103 of pSp1-778c corresponding to the C-terminal end of Sp1. Six overlapping cosmids were isolated containing two exons encoding the zinc fingers and the C-terminus of Sp1. The neomycin targeting vector (pBSp1/Neo) is a promoterless construct containing 2 kb 5' and 3.7 kb 3' regions of homology to the Sp1 gene in pBS II KS. The beta-geo cassette containing a splice acceptor and the picornaviral IRES replaces the 2 kb NcoI fragment with the two exons encoding the zinc fingers and C−terminus of Sp1. |
| Phenotypic information | Sp1-/- embryos survive until day 9.5 (E9.5) of gestation. They are severely retarded in growth and show a broad range of phenotypic abnormalities. Sp1-/- ES cells contribute extensively to every tissue of chimeras until E9.5 but fail to contribute after early embryonic development. |
| Breeding history | The line was backcrossed to C57BL/6 for more than 6 generations. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- Transcription factor Sp1 is essential for early embryonic development but dispensable for cell growth and differentiation.;Marin M, Karis A, Visser P, Grosveld F, Philipsen S, ;1997;Cell;89;619-28; 9160753
- Sp1/Sp3 compound heterozygous mice are not viable: impaired erythropoiesis and severe placental defects.;Krüger Imme, Vollmer Marion, Simmons David G, Elsässer Hans-Peter, Philipsen Sjaak, Suske Guntram, ;2007;Developmental dynamics : an official publication of the American Association of Anatomists;236;2235-44; 17584888