MGI phenotypes (allele matching)
B6.129P2-Sp1tm1Phi/Cnrm
| Status | Available to order |
| EMMA ID | EM:02430 |
| Citation information | RRID:IMSR_EM:02430 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | B6.129P2-Sp1tm1Phi/Cnrm |
| Alternative name | Sp1 knockout |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Sp1tm1Phi |
| Gene/Transgene symbol | Sp1 |
Information from provider
| Provider | Sjaak Philipsen |
| Provider affiliation | Genetics, Erasmus MC |
| Genetic information | A 129 mouse cosmid genomic DNA library was screened with a probe spanning nucleotides 1820-2103 of pSp1-778c corresponding to the C-terminal end of Sp1. Six overlapping cosmids were isolated containing two exons encoding the zinc fingers and the C-terminus of Sp1. The neomycin targeting vector (pBSp1/Neo) is a promoterless construct containing 2 kb 5' and 3.7 kb 3' regions of homology to the Sp1 gene in pBS II KS. The beta-geo cassette containing a splice acceptor and the picornaviral IRES replaces the 2 kb NcoI fragment with the two exons encoding the zinc fingers and C−terminus of Sp1. |
| Phenotypic information | Sp1-/- embryos survive until day 9.5 (E9.5) of gestation. They are severely retarded in growth and show a broad range of phenotypic abnormalities. Sp1-/- ES cells contribute extensively to every tissue of chimeras until E9.5 but fail to contribute after early embryonic development. |
| Breeding history | The line was backcrossed to C57BL/6 for more than 6 generations. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Literature references
- Transcription factor Sp1 is essential for early embryonic development but dispensable for cell growth and differentiation.;Marin M, Karis A, Visser P, Grosveld F, Philipsen S, ;1997;Cell;89;619-28; 9160753
- Sp1/Sp3 compound heterozygous mice are not viable: impaired erythropoiesis and severe placental defects.;Krüger Imme, Vollmer Marion, Simmons David G, Elsässer Hans-Peter, Philipsen Sjaak, Suske Guntram, ;2007;Developmental dynamics : an official publication of the American Association of Anatomists;236;2235-44; 17584888