B6;129S6-Sp140tm1Ptsn/Kctt
| Status | Available to order |
| EMMA ID | EM:09496 |
| International strain name | B6;129S6-Sp140tm1Ptsn/Kctt |
| Alternative name | B6;129S6-Sp140-/- |
| Strain type | Targeted Mutant Strains : Knock-out |
| Allele/Transgene symbol | Sp140tm1.1Ptsn, |
| Gene/Transgene symbol | Sp140 |
Information from provider
| Provider | Mario Saare |
| Provider affiliation | Molecular Pathology group, Institute of Biomedicine and Translational Medicine, University of Tartu |
| Genetic information | The ES cells derived from 129S6/SvEvTac mouse strain were electroporated with a vector containing the neo and TK cassettes for selection, and loxP1 sites that were used to cut out the neo cassette together with exon 9 and partially exon 10. The cloned ES cells were transferred to C57BL/6J blastocysts. |
| Phenotypic information | Sp140 is strongly expressed in B cells. We anticipated the knock-out to have an effect on the levels of different immunoglobulin classes, but the antibody production was unaltered. Also, we tested the avidity of ovalbumin-specific antibodies, but no changes could be determined. B cell population numbers and proportions were normal. The animals are fertile and age normally. |
| Breeding history | The strain has not been backcrossed. The mice have been inbred for five generations. |
| References | None available |
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous C57BL/6J |