B6.Cg-Ldlrtm1Her Tg(APOB*Q2153L)#Boren/Kctt

Status

Available to order

EMMA IDEM:09689
International strain nameB6.Cg-Ldlrtm1Her Tg(APOB*Q2153L)#Boren/Kctt
Alternative name B6.C57BL/6XSJL-Tg(huB100tm).129S7-Ldlrtm1Her
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolLdlrtm1Her,
Gene/Transgene symbolLdlr

Information from provider

ProviderJan Borén
Provider affiliationMolecular and Clinical Medicine, University of Gothenburg
Additional ownerGöran Hansson, Daniel Ketelhuth and Alexandra Bäcklund, The Cardiovascular Medicine Unit, Department of Medicine, Karolinska Institutet, Stockholm, Sweden
Genetic informationThese mice carry the full-length human APOB100 gene, in which codon 2153 has been converted from leucine to glutamine to prevent the formation of ApoB48, generating only ApoB100. They are also deficient in LDLr and have an increased level of LDL-cholesterol, VLDL-cholesterol, and low HDL relative to wild-type mice and develop spontaneous atherosclerosis.
Phenotypic informationHomozygous:
Mice have hypercholesterolemia and develop spontaneous atherosclerotic lesions. Females have an earlier onset with lesions at 18 weeks compared with males (onset at 26 weeks). Mice are healthy and have no visible effect with a normal life span.

Heterozygous:
Mice have mild hypercholesterolemia but do not develop spontaneous atherosclerotic lesions. Mice are healthy and have no visible effect with a normal life span.
Breeding historyMice were received at 4 generations backcrossed to C57BL/6J. The mice were then crossed a further 6 generations to C57BL/6J, purchased from JAX Mice in USA.
References
  • Inhibition of T cell response to native low-density lipoprotein reduces atherosclerosis.;Hermansson Andreas, Ketelhuth Daniel F J, Strodthoff Daniela, Wurm Marion, Hansson Emil M, Nicoletti Antonino, Paulsson-Berne Gabrielle, Hansson Göran K, ;2010;The Journal of experimental medicine;207;1081-93; 20439543
  • Immunotherapy with tolerogenic apolipoprotein B-100-loaded dendritic cells attenuates atherosclerosis in hypercholesterolemic mice.;Hermansson Andreas, Johansson Daniel K, Ketelhuth Daniel F J, Andersson John, Zhou Xinghua, Hansson Göran K, ;2011;Circulation;123;1083-91; 21357823
  • Identification of the low density lipoprotein receptor-binding site in apolipoprotein B100 and the modulation of its binding activity by the carboxyl terminus in familial defective apo-B100.;Boren J, Lee I, Zhu W, Arnold K, Taylor S, Innerarity T L, ;1998;The Journal of clinical investigation;101;1084-93; 9486979
Homozygous fertileyes
Homozygous viableyes
Homozygous matings requiredno
Immunocompromisedno

Information from EMMA

Archiving centreKarolinska Institutet, Stockholm, Sweden
Animals used for archivinghomozygous C57BL/6J

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

MGI phenotypes (allele matching)
  • increased circulating triglyceride level / MGI
  • increased circulating cholesterol level / MGI
  • hyperglycemia / MGI
  • increased circulating LDL cholesterol level / MGI
  • alopecia / MGI
  • abnormal liver physiology / MGI
  • abnormal adrenal gland morphology / MGI
  • scaly skin / MGI
  • thick skin / MGI
  • increased circulating HDL cholesterol level / MGI
  • hepatic steatosis / MGI
  • decreased circulating triglyceride level / MGI
  • increased cholesterol level / MGI
  • increased circulating VLDL cholesterol level / MGI
  • atherosclerotic lesions / MGI
  • homeostasis/metabolism phenotype / MGI
  • increased liver cholesterol level / MGI
  • abnormal microglial cell morphology / MGI
  • abnormal circulating cholesterol level / MGI
  • decreased circulating HDL cholesterol level / MGI
  • abnormal triglyceride level / MGI
  • abnormal CNS glial cell morphology / MGI
  • hyperactivity / MGI
  • abnormal spatial learning / MGI
  • increased circulating insulin level / MGI
  • abnormal astrocyte morphology / MGI
  • amyloid beta deposits / MGI
  • increased circulating VLDL triglyceride level / MGI
  • impaired glucose tolerance / MGI
  • abnormal fat pad morphology / MGI
  • increased susceptibility to atherosclerosis / MGI
  • increased susceptibility to weight gain / MGI
  • increased percent body fat/body weight / MGI
  • abnormal spatial working memory / MGI
  • increased circulating tumor necrosis factor level / MGI
  • decreased circulating interleukin-10 level / MGI
  • increased circulating interleukin-6 level / MGI
  • increased circulating interleukin-1 beta level / MGI
  • increased liver triglyceride level / MGI
  • increased grip strength / MGI
  • decreased susceptibility to weight gain / MGI
  • abnormal hippocampus morphology / MGI
  • obese / MGI
  • increased circulating free fatty acid level / MGI
  • liver inflammation / MGI
  • abnormal glucose homeostasis / MGI
  • decreased circulating corticosterone level / MGI
  • increased circulating alanine transaminase level / MGI
  • increased liver weight / MGI
  • liver fibrosis / MGI
  • oxidative stress / MGI
  • maternal effect / MGI
  • increased hepatocyte apoptosis / MGI
  • decreased lean body mass / MGI
  • abnormal arteriole morphology / MGI
  • abnormal retinal pigment epithelium morphology / MGI
  • abnormal Bruch membrane morphology / MGI
  • abnormal circulating amino acid level / MGI
  • insulin resistance / MGI
  • increased circulating glucose level / MGI
  • abnormal choriocapillaris morphology / MGI
  • abnormal short term spatial reference memory / MGI
  • abnormal synaptic bouton morphology / MGI
  • photoreceptor outer segment degeneration / MGI
  • abnormal synapse morphology / MGI
  • abnormal circulating LDL cholesterol level / MGI
  • abnormal lipid homeostasis / MGI
  • abnormal cholesterol homeostasis / MGI
  • abnormal circulating protein level / MGI

Literature references

  • Inhibition of T cell response to native low-density lipoprotein reduces atherosclerosis.;Hermansson Andreas, Ketelhuth Daniel F J, Strodthoff Daniela, Wurm Marion, Hansson Emil M, Nicoletti Antonino, Paulsson-Berne Gabrielle, Hansson Göran K, ;2010;The Journal of experimental medicine;207;1081-93; 20439543
  • Immunotherapy with tolerogenic apolipoprotein B-100-loaded dendritic cells attenuates atherosclerosis in hypercholesterolemic mice.;Hermansson Andreas, Johansson Daniel K, Ketelhuth Daniel F J, Andersson John, Zhou Xinghua, Hansson Göran K, ;2011;Circulation;123;1083-91; 21357823
  • Identification of the low density lipoprotein receptor-binding site in apolipoprotein B100 and the modulation of its binding activity by the carboxyl terminus in familial defective apo-B100.;Boren J, Lee I, Zhu W, Arnold K, Taylor S, Innerarity T L, ;1998;The Journal of clinical investigation;101;1084-93; 9486979
  • Liver damage promotes pro-inflammatory T-cell responses against apolipoprotein B-100.;Plochg Bastian F J, Englert Hanna, Rangaswamy Chandini, Konrath Sandra, Malle Mandy, Lampalzer Sibylle, Beisel Claudia, Wollin Salma, Frye Maike, Aberle Jens, Kluwe Johannes, Renné Thomas, Mailer Reiner K, ;2022;Journal of internal medicine;291;648-664; 34914849

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Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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