MGI phenotypes (allele matching)
FVB.129P2-Igf2rtm2Wag/Biat
| Status | Available to order |
| EMMA ID | EM:09897 |
| International strain name | FVB.129P2-Igf2rtm2Wag/Biat |
| Alternative name | FVB.129P2-Airn-R2D (FVB.129P2- Igf2r |
| Strain type | Targeted Mutant Strains : Other targeted |
| Allele/Transgene symbol | Igf2rtm2Wag, |
| Gene/Transgene symbol | Igf2r |
Information from provider
| Provider | Denise Barlow |
| Provider affiliation | CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences |
| Genetic information | The R2D allele has a 3.6kb deletion (chr17:12,740,792-12,744,359, GRCm38/mm10) that includes the Airn promoter and the imprint control element (ICE) of the Igf2r imprinted cluster, with 95 bp vector remaining, including a single loxP site. The deletion was created in E14 ES cells (129P2/OlaHsd), which were then used to make chimeras with C57BL/6 blastocysts. The allele has been backcrossed to FVB/N for over 50 generations. |
| Phenotypic information | Homozygous:Homozygous R2D mice are viable and fertile, but show reduced embryonic and neonatal growth due to biallelic expression of Igf2r due to the deletion of the Airn promoter and loss of imprinted silencing of Igf2r on the paternal allele.Heterozygous:Heterozygous R2D mice are viable and fertile, but mice that inherit the deletion paternally have a loss of Igf2r imprinted expression and the reduced growth phenotype described for the homozygous mice. Mice that inherit the R2D allele maternally show no phenotype. |
| Breeding history | R2D mice were derived from 129/Sv x C57BL/6 chimeras and then backcrossed to FVB/N for over 50 generations. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous FVB/N |
Disease and phenotype information
Literature references
- Non-imprinted Igf2r expression decreases growth and rescues the Tme mutation in mice.;Wutz A, Theussl H C, Dausman J, Jaenisch R, Barlow D P, Wagner E F, ;2001;Development (Cambridge, England);128;1881-7; 11311167
- Bidirectional action of the Igf2r imprint control element on upstream and downstream imprinted genes.;Zwart R, Sleutels F, Wutz A, Schinkel A H, Barlow D P, ;2001;Genes & development;15;2361-6; 11562346
- Airn transcriptional overlap, but not its lncRNA products, induces imprinted Igf2r silencing.;Latos Paulina A, Pauler Florian M, Koerner Martha V, Şenergin H Başak, Hudson Quanah J, Stocsits Roman R, Allhoff Wolfgang, Stricker Stefan H, Klement Ruth M, Warczok Katarzyna E, Aumayr Karin, Pasierbek Pawel, Barlow Denise P, ;2012;Science (New York, N.Y.);338;1469-72; 23239737