FVB.129P2-AIDup
| Status | Available to order |
| EMMA ID | EM:09900 |
| International strain name | FVB.129P2-AIDup |
| Alternative name | FVB.129P2-AIDup |
| Strain type | Targeted Mutant Strains : Other targeted |
| Allele/Transgene symbol | Unknown at present |
| Gene/Transgene symbol | Airn to Igf2r |
Information from provider
| Provider | Denise Barlow |
| Provider affiliation | CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences |
| Genetic information | The AIDup allele has a 23kb duplication on chromosome 17 between 3kb downstream of the Airn transcription start site (TSS) (Igf2r intron 2) and 1.9kb downstream of the Igf2r TSS (GRCm38/mm10, chr17: 12,744,358 -12,767,833). This results in an extra 23kb of Airn transcription before it reaches the Igf2r promoter, and duplication of exon 2 of Igf2r that is predicted to cause a frame-shift. This allele was made by chromosome engineering by targeted meiotic recombination (Herault et al 1998), by breeding together the Airn truncation allele (FVB.129P2-AirnT, Sleutels et al 2002 Nature) and the Igf2r promoter deletion (FVB.129P2-IPD, Sleutels et al 2003 EMBO J.) with Hprt-Cre (B6.129S1-Hprt-Cre, Tang et al 2002 Genesis), and screening the offspring of triple mutant males for recombination events. The AIDup allele should have a 129P2 background near the deletion, and a B6/FVB mixed background in other regions of the genome. The background should be closer to FVB as the mice have been backcrossed to FVB/N for 3 generations. |
| Phenotypic information | Homozygous:Homozygous mice should be embryonic lethal between E13.5 and E18.5 due to the predicted frameshift in Igf2r in the duplication (Barlow et al 1991 Nature).Heterozygous:Heterozygotes with a maternally inherited AIDup allele should be embryonic lethal between E13.5 and E18.5 due to the predicted frameshift in Igf2r, while mice with a paternal inheritance of the duplication are viable and fertile. |
| Breeding history | AIDup mice were derived over 2 generations by breeding together FVB.129P2-AirnT, FVB.129P2-IPD and B6.129S1-Hprt-Cre mice, and then screening the offspring of triple mutant males for recombination events, as described in the “Genetic Description” section. The AIDup founder was then backcrossed to FVB/N for 3 generations. |
| References | None available |
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous FVB/N |