FVB.129P2-AIDup
| Status | Available to order |
| EMMA ID | EM:09900 |
| Citation information | RRID:IMSR_EM:09900 Research Resource Identifiers (RRID) are persistent unique ID numbers assigned to help researchers cite key resources (e.g. antibodies, model organisms and software projects) in the biomedical literature to improve transparency and reproducibility in research. See https://www.rrids.org/ for more information. |
| International strain name | FVB.129P2-AIDup |
| Alternative name | FVB.129P2-AIDup |
| Strain type | Targeted Mutant Strains : Other targeted |
| Allele/Transgene symbol | Unknown at present |
| Gene/Transgene symbol | Airn to Igf2r |
Information from provider
| Provider | Denise Barlow |
| Provider affiliation | CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences |
| Genetic information | The AIDup allele has a 23kb duplication on chromosome 17 between 3kb downstream of the Airn transcription start site (TSS) (Igf2r intron 2) and 1.9kb downstream of the Igf2r TSS (GRCm38/mm10, chr17: 12,744,358 -12,767,833). This results in an extra 23kb of Airn transcription before it reaches the Igf2r promoter, and duplication of exon 2 of Igf2r that is predicted to cause a frame-shift. This allele was made by chromosome engineering by targeted meiotic recombination (Herault et al 1998), by breeding together the Airn truncation allele (FVB.129P2-AirnT, Sleutels et al 2002 Nature) and the Igf2r promoter deletion (FVB.129P2-IPD, Sleutels et al 2003 EMBO J.) with Hprt-Cre (B6.129S1-Hprt-Cre, Tang et al 2002 Genesis), and screening the offspring of triple mutant males for recombination events. The AIDup allele should have a 129P2 background near the deletion, and a B6/FVB mixed background in other regions of the genome. The background should be closer to FVB as the mice have been backcrossed to FVB/N for 3 generations. |
| Phenotypic information | Homozygous:Homozygous mice should be embryonic lethal between E13.5 and E18.5 due to the predicted frameshift in Igf2r in the duplication (Barlow et al 1991 Nature).Heterozygous:Heterozygotes with a maternally inherited AIDup allele should be embryonic lethal between E13.5 and E18.5 due to the predicted frameshift in Igf2r, while mice with a paternal inheritance of the duplication are viable and fertile. |
| Breeding history | AIDup mice were derived over 2 generations by breeding together FVB.129P2-AirnT, FVB.129P2-IPD and B6.129S1-Hprt-Cre mice, and then screening the offspring of triple mutant males for recombination events, as described in the “Genetic Description” section. The AIDup founder was then backcrossed to FVB/N for 3 generations. |
| References | None available |
| Homozygous fertile | no |
| Homozygous viable | no |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | University of Veterinary Medicine, Vienna, Austria |
| Animals used for archiving | heterozygous FVB/N males |