C57BL/6-Eomestm1.1Twa/Cnbc
| Status | Available to order |
| EMMA ID | EM:10580 |
| International strain name | C57BL/6-Eomestm1.1Twa/Cnbc |
| Alternative name | Eomes-GFP |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Eomestm1.1Twa, |
| Gene/Transgene symbol | Eomes |
Information from provider
| Provider | Thierry Walzer |
| Provider affiliation | Centre International de Recherche en Infectiologie (CIRI) |
| Genetic information | An IRES-GFP reporter cassette was inserted in the 3' untranslated region of Eomes gene. |
| Phenotypic information | Homozygous:EGFP is expressed in Eomes positive cells, without altering Eomes protein expression. Homozygous mice are normal and have a normal immune function.Heterozygous:Eomes positive cells already express high levels of EGFP |
| Breeding history | ES cells (JM8) are C57BL/6N. The mice were backcrossed at least three times to C57BL/6J. |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | CNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain |
| Animals used for archiving | homozygous C57BL/6J, homozygous C57BL/6J |
| Stage of embryos | 2-cell |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Microcephaly-polymicrogyria-corpus callosum agenesis syndrome / Orphanet_171703
Literature references
- T-bet and Eomes instruct the development of two distinct natural killer cell lineages in the liver and in the bone marrow.;Daussy Cécile, Faure Fabrice, Mayol Katia, Viel Sébastien, Gasteiger Georg, Charrier Emily, Bienvenu Jacques, Henry Thomas, Debien Emilie, Hasan Uzma A, Marvel Jacqueline, Yoh Keigyou, Takahashi Satoru, Prinz Immo, de Bernard Simon, Buffat Laurent, Walzer Thierry, ;2014;The Journal of experimental medicine;211;563-77; 24516120