B6.Cg-Tg(KRT14-cre)1Cgn/CgnCnrm

Status

Available to order

EMMA IDEM:01131
International strain nameB6.Cg-Tg(KRT14-cre)1Cgn/CgnCnrm
Alternative nameK14-Cre
Strain typeTransgenic Strains
Allele/Transgene symbolTg(KRT14-cre)1Cgn
Gene/Transgene symbolTg(KRT14-cre)1Cgn

Information from provider

ProviderThomas Krieg
Provider affiliationDepartment of Dermatology, University of Cologne, Dept. Dermatology
Genetic informationThe expression vector pBBEB-K14 contains 2.18 kb of the human keratin 14 promoter (Staggers et al., 1995) and the SV40 polyA signal of the plasmid K14(c)-luc (J. Kudlow, Univ. of Alabama at Birmingham, USA). The nuclear localisation signal (NLS) containing the cre recombinase open reading frame was excised from the vector pGK-cre (K. Rajewsky and W. Mueller, Univ. of Cologne, Germany) and ligated into the vector pBBEBK14 between the K14 promoter and the SV40 polyA signal. For the generation of transgenic mice the K14 promoter-cre-SV40polyA expression cassette was excised and microinjected in fertilized B6xB6D2F2 oocytes.
Phenotypic informationIf inherited from the father, the K14-cre#47 transgene confers tissue specific deletion of loxP-flanked DNA segments in the epidermis, tongue epithelium and thymic epithelium. No deletion is observed in the esophagus or in the stomach. If the K14cre#47 transgene is inherited from the mother, a dominant maternal phenotype is observed: deletion of loxP-flanked DNA segments occurs in all of the offsprings tissues, even in those littermates that did not inherit the transgene from the mother. This indicates that the transgene is expressed in the oocytes and that the protein is still active when the transgene is lost during meiosis. The stability of the protein is sufficient to confer deletion of paternally inherited loxP-flanked DNA segments after fertilization.
Breeding historyGenerated in C57BL/6 x (C57BL/6 x DBA/2)F2 oocytes; backcrossed to C57BL/6 (4 generations)
References
  • Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein.;Hafner Martin, Wenk Jutta, Nenci Arianna, Pasparakis Manolis, Scharffetter-Kochanek Karin, Smyth Neil, Peters Thorsten, Kess Daniel, Holtkötter Olaf, Shephard Pierre, Kudlow Jeffrey E, Smola Hans, Haase Ingo, Schippers Angela, Krieg Thomas, Müller Werner, ;2004;Genesis (New York, N.Y. : 2000);38;176-81; 15083518

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Literature references

  • Keratin 14 Cre transgenic mice authenticate keratin 14 as an oocyte-expressed protein.;Hafner Martin, Wenk Jutta, Nenci Arianna, Pasparakis Manolis, Scharffetter-Kochanek Karin, Smyth Neil, Peters Thorsten, Kess Daniel, Holtkötter Olaf, Shephard Pierre, Kudlow Jeffrey E, Smola Hans, Haase Ingo, Schippers Angela, Krieg Thomas, Müller Werner, ;2004;Genesis (New York, N.Y. : 2000);38;176-81; 15083518

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

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Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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