FVB/N-Tg(Nes-rtTA)306Rvs/Cnrm
| Status | Available to order |
| EMMA ID | EM:01183 |
| International strain name | FVB/N-Tg(Nes-rtTA)306Rvs/Cnrm |
| Alternative name | Nes-rtTA |
| Strain type | Transgenic Strains |
| Allele/Transgene symbol | Tg(Nes-rtTA)306Rvs, |
| Gene/Transgene symbol | Tg(Nes-rtTA)306Rvs |
Information from provider
| Provider | Steven A. Reeves |
| Provider affiliation | Massachusetts General Hospital |
| Genetic information | The rtTA gene was inserted downstream of a hybrid promoter containing the rat nestin intron II and thymidine kinase (TK) minimal promoter. The nestin intron II has previously been shown to direct expression of a reporter gene to the developing neuroepithelium and spinal cord of transgenic mice. Immediately downstream of the rtTA gene an internal ribosomal entry site (IRES) was inserted followed by a beta-galactosidase/neomycin fusion gene (beta-geo). The IRES element allows reentry of the ribosomes onto the polycistronic mRNA and therefore translation of the fusion beta-geo transcript. The expression of beta galactosidase activity facilitates detection of the rtTA by X-gal staining in the whole embryo, as well as in embryo sections. |
| Phenotypic information | No phenotype defect detected. Gene expression in the rtTA-containing system is inducible with tetracycline or one of its derivatives, such as doxycycline. In the latter system, the mutant rtTA requires the allosteric effect of tetracycline binding in order to bind the tetO/minimal CMV promoter. Studies carried in cultured cells have indicated that induction of a reporter transgene by the addition of doxycycline in the rtTA-containing Tet system is more rapid then in the tTA-containing system, which depends on the removal of tetracycline for transgene expression. |
| References |
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Information from EMMA
| Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Literature references
- Overexpression of p27Kip1 lengthens the G1 phase in a mouse model that targets inducible gene expression to central nervous system progenitor cells.;Mitsuhashi T, Aoki Y, Eksioglu Y Z, Takahashi T, Bhide P G, Reeves S A, Caviness V S, ;2001;Proceedings of the National Academy of Sciences of the United States of America;98;6435-40; 11371649