STOCK Hrhr Alox12btm1Krie Krt14tm1.1(cre/ERT2)Hjg/KrieH
| Status | Available to order |
| EMMA ID | EM:12462 |
| International strain name | STOCK Hrhr Alox12btm1Krie Krt14tm1.1(cre/ERT2)Hjg/KrieH |
| Alternative name | SKH1/Alox12bflox/K14CreJ |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Krt14tm1.1(cre/ERT2)Hjg, |
| Gene/Transgene symbol | Krt14 |
Information from provider
| Provider | Peter Krieg |
| Provider affiliation | Deutsches Krebsforschungszentrum |
| Additional owner | Prof. Pierre Chambon, IGBMC, Illkirch, France |
| Genetic information | 1. Generation of mice harboring loxP containing Alox12b alleles (Epp et al., 2003): by using a 7.4-kb BamHI fragment from a 129/Ola mouse genomic PAC library and a 4.4-kb PCR-generated fragment containing exon 7–12, a homology region was cloned into pBluescript KS+ that spanned from 226 nt upstream of exon 3 to 1081 nt downstream of exon 11. A tk/neo selection cassette flanked by two loxP sites was inserted 230 bp upstream of exon 8, replacing a 222-bp AccI fragment; a third loxP site was inserted 84 bp downstream of exon 8, deleting a BamHI site. The linearized targeting vector was electroporated into ES cells. ES cells were injected into C57BL/6 blastocysts to generate chimeric mice, which were mated with C57BL/6 females. F1 heterozygotes (Alox12b+/flox) were then crossed to 129S6. 2. Generation of conditional Alox12b knockout mice: mice harboring loxP containing Alox12b alleles were crossed with CMV-cre deleter mice to remove Tk/neo resistance cassette and to obtain Alox12b+/fl mice in which exon 8 of Alox12b is flanked by two loxP sites. Alox12bfl/fl mice were crossed to mice expressing cre recombinase fused to the human estrogen receptor under the control of a truncated keratin 14 promoter (Amen et al., 2013) to obtain mice with Tamoxifen-inducible, epidermis-specific deletion of Alox12b (Alox12bfl/fl K14CreJ mice). The Alox12bfl/fl K14CreJ mice were backcrossed to the hairless SKH1 background for at least five generations. |
| Phenotypic information | Homozygous:Complete ablation of Alox12b in epidermis and other keratin 14-expressing tissues.Heterozygous:Ablation of one copy of Alox12b allele in epidermis and other keratin 14-expressing tissues. |
| Breeding history | This line was originally 129S6 but later it was back-crossed for about five generations to SKH1 (Hrhr). |
| References |
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| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- Congenital non-bullous ichthyosiform erythroderma / Orphanet_79394
- Self-improving collodion baby / Orphanet_281122
- Lamellar ichthyosis / Orphanet_313
- Naegeli-Franceschetti-Jadassohn syndrome / Orphanet_69087
- Epidermolysis bullosa simplex, generalized intermediate / Orphanet_79399
- Epidermolysis bullosa simplex, autosomal recessive K14 / Orphanet_89838
- Epidermolysis bullosa simplex, generalized severe / Orphanet_79396
- Epidermolysis bullosa simplex with mottled pigmentation / Orphanet_79397
- Dermatopathia pigmentosa reticularis / Orphanet_86920
- Localized epidermolysis bullosa simplex / Orphanet_79400
Literature references
- The role of lipoxygenases in epidermis.;Krieg Peter, Fürstenberger Gerhard, ;2014;Biochimica et biophysica acta;1841;390-400; 23954555