- enlarged spleen / MGI
B6.Cg-Gt(ROSA)26Sortm1(Trib1,-EGFP)Ekt Lyz2tm1(cre)Ifo/H
Status | Available to order |
EMMA ID | EM:14886 |
International strain name | B6.Cg-Gt(ROSA)26Sortm1(Trib1,-EGFP)Ekt Lyz2tm1(cre)Ifo/H |
Alternative name | Trib1 Rosa TG |
Strain type | Targeted Mutant Strains : Knock-in |
Allele/Transgene symbol | Lyz2tm1(cre)Ifo, |
Gene/Transgene symbol | Lyz2 |
Information from provider
Provider | Endre Kiss-Toth |
Provider affiliation | IICD, University of Sheffield |
Genetic information | Murine Trib1 complementary DNA was introduced into the previously described pROSA26, loxP-flanked STOP and Frt-flanked internal ribosomal entry site–eGFP targeting construct and then inserted into the ubiquitously expressed Gt(ROSA)26Sor locus of Bruce 4 (B6.Cg-Thy1a origin) mouse ES cells by homologous recombination; correct integration was confirmed by Southern blotting. The cre recombinase-mediated excision of the STOP cassette produces a bicistronic Trib1-eGFP transcript. The presently submitted strain results from further crossing to a Lyz2-cre recombinase-deleter strain, and it still carries the Lyz2-cre deleter construct, so that the re-animated line can be effectively used for het-het breeding to generate experimental and control littermates. |
Phenotypic information | Homozygous:No overt phenotype.Heterozygous:No overt phenotype. |
References |
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Homozygous fertile | yes |
Homozygous viable | yes |
Homozygous matings required | no |
Immunocompromised | no |
Information from EMMA
Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
Disease and phenotype information
MGI phenotypes (allele matching)
Literature references
- Myeloid Tribbles 1 induces early atherosclerosis via enhanced foam cell expansion.;Johnston Jessica M, Angyal Adrienn, Bauer Robert C, Hamby Stephen, Suvarna S Kim, Baidžajevas Kajus, Hegedus Zoltan, Dear T Neil, Turner Martin, null null, Wilson Heather L, Goodall Alison H, Rader Daniel J, Shoulders Carol C, Francis Sheila E, Kiss-Toth Endre, ;2019;Science advances;5;eaax9183; 31692955
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