- decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
- decreased T cell proliferation / MGI
- abnormal liver morphology / MGI
- decreased acute inflammation / MGI
- decreased tumor necrosis factor secretion / MGI
- decreased interferon-gamma secretion / MGI
- decreased physiological sensitivity to xenobiotic / MGI
- increased bone mineral density / MGI
- abnormal osteoclast physiology / MGI
- abnormal cardiovascular system physiology / MGI
- abnormal inflammatory response / MGI
- decreased susceptibility to viral infection / MGI
- dilated renal tubules / MGI
- decreased heart weight / MGI
- delayed wound healing / MGI
- nervous system phenotype / MGI
- abnormal physiological neovascularization / MGI
- renal tubular necrosis / MGI
- increased osteoclast cell number / MGI
- decreased osteoclast cell number / MGI
- decreased bone resorption / MGI
- abnormal osteoblast physiology / MGI
- abnormal response to infection / MGI
- decreased cardiac muscle contractility / MGI
- increased susceptibility to injury / MGI
- skeleton phenotype / MGI
- hyporesponsive to tactile stimuli / MGI
- increased circulating creatinine level / MGI
- increased blood urea nitrogen level / MGI
- decreased susceptibility to type IV hypersensitivity reaction / MGI
- increased interleukin-10 secretion / MGI
- decreased interleukin-12 secretion / MGI
- increased interleukin-4 secretion / MGI
- decreased sensitivity to induced cell death / MGI
- increased trabecular bone thickness / MGI
- mortality/aging / MGI
- decreased fibroblast chemotaxis / MGI
- increased bone marrow cell number / MGI
- abnormal blood cell morphology/development / MGI
- abnormal bone mineralization / MGI
- increased hematopoietic stem cell number / MGI
- increased sensitivity to induced morbidity/mortality / MGI
B6Rcc.129-Cd44tm2.1Ugu Spp1tm1Rit/Cnrm
Status | Available to order |
EMMA ID | EM:01992 |
International strain name | B6Rcc.129-Cd44tm2.1Ugu Spp1tm1Rit/Cnrm |
Alternative name | C57 BL/6RCC CD44v10-/- OPN-/- |
Strain type | Targeted Mutant Strains : Knock-out |
Allele/Transgene symbol | Spp1tm1Rit, |
Gene/Transgene symbol | Spp1 |
Information from provider
Provider | Ursula Günthert |
Provider affiliation | Institut für Pathologie, University of Basel |
Genetic information | The mouse Cd44 variant region was isolated from a 129SV genomic library. Two 34 bp loxP sites were inserted in direct repeats into a single BstEII site 5' of exon v10 and at the 3' end of the neo resistance cassette, which was then inserted into the single BstXI site 3' of exon v10. The targeting vector was transfected in ES cells and homologous recombinant clones injected into C57BL/6 blastocysts. Chimaeric male offspring was then mated with C57BL/6 cre recombinase deleter females to allow the removal of the loxP flanked region. Offspring was genotyped by PCR using exon v10 flanking oligos and analysing for the deletion of the loxP targeted region. The neomycin cassette from pMC1neo was inserted into the EagI site in exon 6 of the Spp1 (secreted phosphoprotein 1; formerly: Opn, osteopontin) gene (a 4.8 kb fragment from 129 mice), that had been cloned into pBluescript (Rittling et al., 1998). |
Phenotypic information | Cd44v10-/-: Strongly reduced symptoms in autoimmune diseases. Opn (Spp1)-/-: Strongly reduced symptoms in autoimmune diseases and impaired bone remodeling. |
Breeding history | 10 generations of backcross to C57BL/6Rcc. Currently bred as C57BL/6Rcc Cd44v10-/- Opn-/- |
References |
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Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
MGI phenotypes (allele matching)
Literature references
- Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
- The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease.;Chabas D, Baranzini S E, Mitchell D, Bernard C C, Rittling S R, Denhardt D T, Sobel R A, Lock C, Karpuj M, Pedotti R, Heller R, Oksenberg J R, Steinman L, ;2001;Science (New York, N.Y.);294;1731-5; 11721059
- Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro.;Rittling S R, Matsumoto H N, McKee M D, Nanci A, An X R, Novick K E, Kowalski A J, Noda M, Denhardt D T, ;1998;Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research;13;1101-11; 9661074
- Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival.;Denhardt D T, Noda M, O'Regan A W, Pavlin D, Berman J S, ;2001;The Journal of clinical investigation;107;1055-61; 11342566
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