B6Rcc.129S7-Spp1tm1Rit/Cnrm

Status

Available to order

EMMA IDEM:01993
International strain nameB6Rcc.129S7-Spp1tm1Rit/Cnrm
Alternative nameC57 BL/6RCC OPN-/-
Strain typeTargeted Mutant Strains : Knock-out
Allele/Transgene symbolSpp1tm1Rit,
Gene/Transgene symbolSpp1

Information from provider

ProviderUrsula Günthert
Provider affiliationInstitut für Pathologie, University of Basel
Genetic informationThe neomycin cassette from pMC1neo was inserted into the EagI site in exon 6 of the Spp1 (secreted phosphoprotein 1; formerly: Opn, osteopontin) gene (a 4.8 kb fragment from 129 mice), that had been cloned into pBluescript (Rittling et al., 1998).
Phenotypic informationStrongly reduced symptoms in autoimmune diseases and impaired bone remodeling.
References
  • The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease.;Chabas D, Baranzini S E, Mitchell D, Bernard C C, Rittling S R, Denhardt D T, Sobel R A, Lock C, Karpuj M, Pedotti R, Heller R, Oksenberg J R, Steinman L, ;2001;Science (New York, N.Y.);294;1731-5; 11721059
  • Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro.;Rittling S R, Matsumoto H N, McKee M D, Nanci A, An X R, Novick K E, Kowalski A J, Noda M, Denhardt D T, ;1998;Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research;13;1101-11; 9661074
  • Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival.;Denhardt D T, Noda M, O'Regan A W, Pavlin D, Berman J S, ;2001;The Journal of clinical investigation;107;1055-61; 11342566

Information from EMMA

Archiving centreCNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

MGI phenotypes (allele matching)
  • abnormal liver morphology / MGI
  • decreased acute inflammation / MGI
  • decreased tumor necrosis factor secretion / MGI
  • decreased interferon-gamma secretion / MGI
  • decreased physiological sensitivity to xenobiotic / MGI
  • increased bone mineral density / MGI
  • abnormal osteoclast physiology / MGI
  • abnormal cardiovascular system physiology / MGI
  • abnormal inflammatory response / MGI
  • decreased susceptibility to viral infection / MGI
  • dilated renal tubules / MGI
  • decreased heart weight / MGI
  • delayed wound healing / MGI
  • nervous system phenotype / MGI
  • abnormal physiological neovascularization / MGI
  • renal tubular necrosis / MGI
  • increased osteoclast cell number / MGI
  • decreased osteoclast cell number / MGI
  • decreased bone resorption / MGI
  • abnormal osteoblast physiology / MGI
  • abnormal response to infection / MGI
  • decreased cardiac muscle contractility / MGI
  • increased susceptibility to injury / MGI
  • skeleton phenotype / MGI
  • hyporesponsive to tactile stimuli / MGI
  • increased circulating creatinine level / MGI
  • increased blood urea nitrogen level / MGI
  • decreased susceptibility to type IV hypersensitivity reaction / MGI
  • increased interleukin-10 secretion / MGI
  • decreased interleukin-12 secretion / MGI
  • increased interleukin-4 secretion / MGI
  • decreased sensitivity to induced cell death / MGI
  • increased trabecular bone thickness / MGI
  • mortality/aging / MGI
  • decreased fibroblast chemotaxis / MGI
  • increased bone marrow cell number / MGI
  • abnormal blood cell morphology/development / MGI
  • abnormal bone mineralization / MGI
  • increased hematopoietic stem cell number / MGI
  • increased sensitivity to induced morbidity/mortality / MGI

Literature references

  • The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease.;Chabas D, Baranzini S E, Mitchell D, Bernard C C, Rittling S R, Denhardt D T, Sobel R A, Lock C, Karpuj M, Pedotti R, Heller R, Oksenberg J R, Steinman L, ;2001;Science (New York, N.Y.);294;1731-5; 11721059
  • Mice lacking osteopontin show normal development and bone structure but display altered osteoclast formation in vitro.;Rittling S R, Matsumoto H N, McKee M D, Nanci A, An X R, Novick K E, Kowalski A J, Noda M, Denhardt D T, ;1998;Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research;13;1101-11; 9661074
  • Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival.;Denhardt D T, Noda M, O'Regan A W, Pavlin D, Berman J S, ;2001;The Journal of clinical investigation;107;1055-61; 11342566

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

  • Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740*
  • Rederivation of mice from frozen stock, delivery time available upon request . €3880*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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