B6Rcc.Cg-Ptprca Cd44tm2.1Ugu/Cnrm
Status | Available to order |
EMMA ID | EM:01997 |
International strain name | B6Rcc.Cg-Ptprca Cd44tm2.1Ugu/Cnrm |
Alternative name | C57 BL/6RCC CD44v10-/- Ly5.1 |
Strain type | Spontaneous |
Allele/Transgene symbol | Ptprca, |
Gene/Transgene symbol | Ptprc |
Information from provider
Provider | Ursula Günthert |
Provider affiliation | Institut für Pathologie, University of Basel |
Genetic information | The mouse Cd44 variant region was isolated from a 129SV genomic library. Two 34 bp loxP sites were inserted in direct repeats into a single BstEII site 5' of exon v10 and at the 3' end of the neo resistance cassette, which was then inserted into the single BstXI site 3' of exon v10. The targeting vector was transfected in ES cells and homologous recombinant clones injected into C57BL/6 blastocysts. Chimaeric male offspring was then mated with C57BL/6 cre recombinase deleter females to allow the removal of the loxP flanked region. Offspring was genotyped by PCR using exon v10 flanking oligos and analysing for the deletion of the loxP targeted region. Ptprca (or Cd45.1 or Ly5.1): spontaneous mutation in the Ptprc (Cd45) gene, distinguishing it from Cd45.2 congenic strains. |
Phenotypic information | Cd44v10-/-: Strongly reduced symptoms in autoimmune diseases and haemopoiesis. Ptprca (or Cd45.1 or Ly5.1): used as congenic marker for transplant studies. |
Breeding history | 10 generations backcrossed to C57BL/6Rcc. Currently bred as C57BL/6Rcc Cd44v10-/- Ly5.1 |
References |
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Information from EMMA
Archiving centre | CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy |
Disease and phenotype information
Orphanet associated rare diseases, based on orthologous gene matching
- T-B+ severe combined immunodeficiency due to CD45 deficiency / Orphanet_169157
MGI phenotypes (allele matching)
Literature references
- Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330
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