CByIco.129-Rag2^tm1Fwa Cd44^tm1.1Ugu/H

Status	Available to order
EMMA ID	EM:02022
International strain name	CByIco.129-Rag2^tm1Fwa Cd44^tm1.1Ugu/H
Alternative name	BALB/cByJIco Rag2-/- CD44v6v7-/-
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Cd44^tm1.1Ugu, Rag2^tm1Fwa
Gene/Transgene symbol	Cd44, Rag2

Information from provider

Provider	Ursula Günthert
Provider affiliation	Institut für Pathologie, University of Basel
Genetic information	A loxP site was inserted 3' of exon v6 and a neomycin resistance cassette, with a 5' loxP site, was inserted 5' of exon v6 of the Cd44 gene. During insertion, 1.6 kb of sequence was lost in the exon v7 region; cre recombinase was transiently expressed in ES cells allowing the removal of neomycin cassette and exon v6. Mice lack both exon v6 and v7. Insertion of a neomycin resistance cassette into the unique exon of the Rag2 gene by homologous recombination leading to the deletion of 286 amino acids.
Phenotypic information	Rag2-/-: No mature T and B cells. Cd44v6v7-/-: Strongly reduced symptoms in autoimmune diseases.
References	RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487 Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	homozygous BALB/cByJIco, homozygous BALB/cByJIco
Breeding at archiving centre	Maintained by intercrossing double homozygotes prior to and during archiving
Stage of embryos	2-cell

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Severe combined immunodeficiency due to complete RAG1/2 deficiency / Orphanet_331206
Omenn syndrome / Orphanet_39041
Combined immunodeficiency with granulomatosis / Orphanet_157949

MGI phenotypes (allele matching)

decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
decreased T cell proliferation / MGI
abnormal digestive system physiology / MGI
immune system phenotype / MGI
abnormal T-helper 1 physiology / MGI
abnormal T-helper 2 physiology / MGI
increased granulocyte number / MGI
abnormal spleen morphology / MGI
spleen hypoplasia / MGI
decreased body size / MGI
arrested B cell differentiation / MGI
decreased IgM level / MGI
thymus hypoplasia / MGI
arrested T cell differentiation / MGI
increased susceptibility to infection / MGI
abnormal effector T cell morphology / MGI
increased natural killer cell mediated cytotoxicity / MGI
decreased double-positive T cell number / MGI
increased macrophage cell number / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
lymph node hypoplasia / MGI
absent mature B cells / MGI
abnormal T cell receptor V(D)J recombination / MGI
abnormal immunoglobulin V(D)J recombination / MGI
decreased thymocyte number / MGI
lung inflammation / MGI
increased susceptibility to bacterial infection / MGI
abnormal macrophage physiology / MGI
colitis / MGI
abnormal response to transplant / MGI
abnormal interferon secretion / MGI
abnormal chemokine secretion / MGI
decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
abnormal intestinal mucosa morphology / MGI
abnormal B cell morphology / MGI
decreased susceptibility to parasitic infection / MGI
decreased double-negative T cell number / MGI
increased double-negative T cell number / MGI
decreased susceptibility to type IV hypersensitivity reaction / MGI
abnormal stomach mucosa morphology / MGI
abnormal intestinal epithelium morphology / MGI
abnormal B cell number / MGI
decreased pre-B cell number / MGI
decreased mature B cell number / MGI
increased immature B cell number / MGI
decreased immature B cell number / MGI
abnormal gamma-delta T cell differentiation / MGI

MGI phenotypes (gene matching)

decreased mast cell number / MGI
altered response to myocardial infarction / MGI
abnormal cell morphology / MGI
decreased mast cell histamine storage / MGI
abnormal uterus morphology / MGI
thin epidermis / MGI
abnormal lipid level / MGI
abnormal vasculogenesis / MGI
abnormal digestive system physiology / MGI
impaired wound healing / MGI
impaired macrophage phagocytosis / MGI
liver inflammation / MGI
lung inflammation / MGI
abnormal definitive hematopoiesis / MGI
no abnormal phenotype detected / MGI
abnormal CD4-positive, alpha beta T cell morphology / MGI
abnormal neutrophil physiology / MGI
short tibia / MGI
impaired skin barrier function / MGI
delayed wound healing / MGI
decreased myocardial infarction size / MGI
abnormal leukocyte migration / MGI
peritoneal inflammation / MGI
decreased susceptibility to induced arthritis / MGI
decreased tumor growth/size / MGI
abnormal leukocyte adhesion / MGI
abnormal physiological neovascularization / MGI
increased susceptibility to induced arthritis / MGI
enhanced coordination / MGI
decreased cholesterol level / MGI
abnormal vascular endothelial cell physiology / MGI
decreased susceptibility to kidney reperfusion injury / MGI
abnormal miniature excitatory postsynaptic currents / MGI
decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
increased mean systemic arterial blood pressure / MGI
abnormal CD8-positive, alpha beta T cell morphology / MGI
abnormal response to infection / MGI
increased susceptibility to parasitic infection / MGI
impaired natural killer cell mediated cytotoxicity / MGI
decreased acute inflammation / MGI
increased acute inflammation / MGI
decreased T cell proliferation / MGI
digestive/alimentary phenotype / MGI
immune system phenotype / MGI
hematopoietic system phenotype / MGI
abnormal CD4-positive, alpha-beta T cell physiology / MGI
abnormal T-helper 1 physiology / MGI
abnormal T-helper 2 physiology / MGI
choroidal neovascularization / MGI
decreased circulating creatinine level / MGI
decreased blood urea nitrogen level / MGI
abnormal vascular smooth muscle physiology / MGI
decreased angiogenesis / MGI
decreased susceptibility to type IV hypersensitivity reaction / MGI
abnormal cell physiology / MGI
abnormal vascular endothelial cell morphology / MGI
abnormal involution of the mammary gland / MGI
increased diameter of tibia / MGI
abnormal osteoclast differentiation / MGI
increased circulating tumor necrosis factor level / MGI
decreased circulating tumor necrosis factor level / MGI
increased tumor necrosis factor secretion / MGI
abnormal interleukin secretion / MGI
increased circulating interferon-gamma level / MGI
increased circulating interleukin-2 level / MGI
increased interleukin-1 beta secretion / MGI
increased interleukin-10 secretion / MGI
increased interleukin-6 secretion / MGI
impaired neutrophil recruitment / MGI
abnormal chemokine secretion / MGI
decreased transforming growth factor level / MGI
decreased sensitivity to induced cell death / MGI
abnormal epidermal lamellar body morphology / MGI
increased sensitivity to induced morbidity/mortality / MGI
abnormal circulating chemokine level / MGI
abnormal circulating cytokine level / MGI
decreased activation-induced cell death of T cells / MGI
increased mitochondria size / MGI
abnormal mitochondrial crista morphology / MGI
decreased fibroblast proliferation / MGI
impaired leukocyte tethering or rolling / MGI
decreased monocyte cell number / MGI
increased granulocyte number / MGI
decreased bone marrow cell number / MGI
alopecia / MGI
abnormal digestive system morphology / MGI
abnormal intestinal epithelium morphology / MGI
abnormal intestinal mucosa morphology / MGI
abnormal spleen morphology / MGI
small spleen / MGI
spleen hypoplasia / MGI
abnormal Peyer's patch morphology / MGI
abnormal thymus morphology / MGI
decreased thymocyte number / MGI
abnormal immune system cell morphology / MGI
decreased body size / MGI
abnormal humoral immune response / MGI
arrested B cell differentiation / MGI
decreased IgG level / MGI
decreased IgM level / MGI
decreased IgA level / MGI
thymus hypoplasia / MGI
arrested T cell differentiation / MGI
lung inflammation / MGI
neoplasm / MGI
abnormal B cell differentiation / MGI
no abnormal phenotype detected / MGI
small lymph nodes / MGI
abnormal lymph node morphology / MGI
abnormal lymphopoiesis / MGI
abnormal pre-B cell morphology / MGI
increased susceptibility to infection / MGI
abnormal double-negative T cell morphology / MGI
abnormal double-positive T cell morphology / MGI
increased susceptibility to bacterial infection / MGI
abnormal effector T cell morphology / MGI
abnormal macrophage physiology / MGI
abnormal B cell number / MGI
abnormal B cell physiology / MGI
abnormal immune system organ morphology / MGI
colitis / MGI
absent Peyer's patches / MGI
increased natural killer cell mediated cytotoxicity / MGI
erythroderma / MGI
abnormal lymphocyte physiology / MGI
abnormal B cell morphology / MGI
decreased lymphocyte cell number / MGI
decreased B cell number / MGI
decreased T cell number / MGI
decreased susceptibility to parasitic infection / MGI
decreased double-negative T cell number / MGI
increased double-negative T cell number / MGI
decreased double-positive T cell number / MGI
decreased T cell proliferation / MGI
cachexia / MGI
increased macrophage cell number / MGI
abnormal T-helper 1 physiology / MGI
decreased susceptibility to type IV hypersensitivity reaction / MGI
abnormal response to transplant / MGI
abnormal T cell morphology / MGI
decreased NK T cell number / MGI
decreased CD4-positive, alpha beta T cell number / MGI
decreased CD8-positive, alpha-beta T cell number / MGI
lymph node hypoplasia / MGI
decreased pre-B cell number / MGI
decreased mature B cell number / MGI
absent mature B cells / MGI
increased immature B cell number / MGI
decreased immature B cell number / MGI
abnormal gamma-delta T cell differentiation / MGI
decreased spleen white pulp amount / MGI
abnormal interferon secretion / MGI
abnormal chemokine secretion / MGI
abnormal T cell receptor V(D)J recombination / MGI
abnormal immunoglobulin V(D)J recombination / MGI
absent Hassall's corpuscle / MGI
small inguinal lymph nodes / MGI
decreased susceptibility to bacterial infection induced morbidity/mortality / MGI
increased DN3 thymocyte number / MGI
abnormal stomach mucosa morphology / MGI
small cervical lymph nodes / MGI

Literature references

RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement.;Shinkai Y, Rathbun G, Lam K P, Oltz E M, Stewart V, Mendelsohn M, Charron J, Datta M, Young F, Stall A M, ;1992;Cell;68;855-67; 1547487
Abrogation of experimental colitis correlates with increased apoptosis in mice deficient for CD44 variant exon 7 (CD44v7).;Wittig B M, Johansson B, Zöller M, Schwärzler C, Günthert U, ;2000;The Journal of experimental medicine;191;2053-64; 10859330

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Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
Tissue - Types of tissue, service fee and delivery time available upon request

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CByIco.129-Rag2tm1Fwa Cd44tm1.1Ugu/H