B6.129-Tnfrsf1b^tm1Mwm Tnfrsf1a^tm1Blt/Cnrm

Status	Available to order
EMMA ID	EM:02532
International strain name	B6.129-Tnfrsf1b^tm1Mwm Tnfrsf1a^tm1Blt/Cnrm
Alternative name	B6.129S-Tnfrsf1aTnfrsf1b
Strain type	Targeted Mutant Strains : Knock-out
Allele/Transgene symbol	Tnfrsf1a^tm1Blt,
Gene/Transgene symbol	Tnfrsf1a

Information from provider

Provider	Horst Bluethmann
Provider affiliation	Pharmaceuticals Divisons, F. Hoffmann-La Roche Ltd.
Genetic information	The double mutant mice lacking both Tnfrsf1a and Tnfrsf1b were generated by intercrossing the singly deficient mice. B6.129S-Tnfrsf1a^tm1Blt: A genomic fragment containing the exons II and III and 5' sequences from exon IV of the Tnfrsf1a gene was replaced by a neomycin resistance gene flanked by phosphoglycerate kinase-1 (pgk-1) promoter- and poly (A)+ signal sequences. The insertion of the neo cassette resulted in the introduction of new BglII and StuI sites which were used to confirm the targeting event. The Tnfrsf1a homologies provided at both sides of the neo cassette were 868 bp and ~ 5 kb, respectively. The targeting vector was linearized with XbaI before electroporation into E14 embryonic stem cells derived from 129/Sv mice. B6.129S-Tnfrsf1b^tm1Mwm: A neomycin resistance gene under the control phosphoglycerate kinase-1 (pgk-1) promoter was inserted into the BstBII site in the second exon, which contains the signal peptide region of TNFR2.
Phenotypic information	B6.129S-Tnfrsf1a^tm1Blt: Mice lacking the tumor necrosis factor receptor 1 are resistant to TNF-mediated toxicity but highly susceptible to infection by Listeria monocytogenes (PMID: 8395024) B6.129S-Tnfrsf1b^tm1Mwm: Mice deficient for Tnfrsf1b show decreased T cell proliferation, decreased interferon-gamma secretion, and decreased interleukin-2 secretion (PMID: 7990930).
Breeding history	The single deficient strains were backcrossed each to C57BL/6 for 6 generations, then intercrossed.
References	Mice lacking the tumour necrosis factor receptor 1 are resistant to TNF-mediated toxicity but highly susceptible to infection by Listeria monocytogenes.;Rothe J, Lesslauer W, Lötscher H, Lang Y, Koebel P, Köntgen F, Althage A, Zinkernagel R, Steinmetz M, Bluethmann H, ;1993;Nature;364;798-802; 8395024 Decreased sensitivity to tumour-necrosis factor but normal T-cell development in TNF receptor-2-deficient mice.;Erickson S L, de Sauvage F J, Kikly K, Carver-Moore K, Pitts-Meek S, Gillett N, Sheehan K C, Schreiber R D, Goeddel D V, Moore M W, ;1994;Nature;372;560-3; 7990930
Homozygous fertile	yes
Homozygous viable	yes
Homozygous matings required	yes
Immunocompromised	yes

Information from EMMA

Archiving centre	CNR, Consiglio Nazionale delle Ricerche, Monterotondo, Italy

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

Tumor necrosis factor receptor 1 associated periodic syndrome / Orphanet_32960
Sézary syndrome / Orphanet_3162
Classic mycosis fungoides / Orphanet_2584

MGI phenotypes (allele matching)

abnormal response/metabolism to endogenous compounds / MGI
decreased circulating interleukin-6 level / MGI
decreased sensitivity to induced morbidity/mortality / MGI
decreased physiological sensitivity to xenobiotic / MGI
decreased sensitivity to xenobiotic induced morbidity/mortality / MGI
abnormal sleep pattern / MGI
impaired central nervous system regeneration / MGI
increased sensitivity to induced cell death / MGI
increased susceptibility to bacterial infection induced morbidity/mortality / MGI
impaired humoral immune response / MGI
decreased susceptibility to bacterial infection / MGI
decreased circulating alanine transaminase level / MGI
abnormal Peyer's patch morphology / MGI
abnormal immune system physiology / MGI
abnormal lymph node B cell domain morphology / MGI
abnormal Peyer's patch follicle morphology / MGI
increased susceptibility to bacterial infection / MGI
decreased susceptibility to experimental autoimmune encephalomyelitis / MGI
increased susceptibility to parasitic infection / MGI
increased susceptibility to type I hypersensitivity reaction / MGI
decreased Peyer's patch number / MGI
absent follicular dendritic cells / MGI
decreased IgG1 level / MGI
decreased interleukin-6 secretion / MGI
decreased T cell proliferation / MGI
decreased interferon-gamma secretion / MGI
decreased interleukin-2 secretion / MGI
increased susceptibility to experimental autoimmune encephalomyelitis / MGI
decreased susceptibility to autoimmune diabetes / MGI
periinsulitis / MGI

Literature references

Mice lacking the tumour necrosis factor receptor 1 are resistant to TNF-mediated toxicity but highly susceptible to infection by Listeria monocytogenes.;Rothe J, Lesslauer W, Lötscher H, Lang Y, Koebel P, Köntgen F, Althage A, Zinkernagel R, Steinmetz M, Bluethmann H, ;1993;Nature;364;798-802; 8395024
Decreased sensitivity to tumour-necrosis factor but normal T-cell development in TNF receptor-2-deficient mice.;Erickson S L, de Sauvage F J, Kikly K, Carver-Moore K, Pitts-Meek S, Gillett N, Sheehan K C, Schreiber R D, Goeddel D V, Moore M W, ;1994;Nature;372;560-3; 7990930

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen embryos. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
For this strain no provider MTA is needed. Distribution is based on the EMMA conditions only.

EMMA conditions
Legally binding conditions for the transfer

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B6.129-Tnfrsf1btm1Mwm Tnfrsf1atm1Blt/Cnrm