B6.129X1-Thtm1(cre)Te/Kieg
| Status | Available to order |
| EMMA ID | EM:00254 |
| International strain name | B6.129X1-Thtm1(cre)Te/Kieg |
| Alternative name | Th-IRES-Cre |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Thtm1(cre)Te, |
| Gene/Transgene symbol | Th |
Information from provider
| Provider | Ted Ebendal |
| Provider affiliation | Department of Neuroscience, Uppsala University |
| Genetic information | The cre recombinase (derived from te pMC-Cre plamid obtained by Klaus Rajewsky) was cloned following an IRES sequence (obtained from Austin Smith). An frt-flanked neomycin selection cassette was added and the construct cloned in the 3' untranslated end of the tyrosine hydroxylase (Th) gene (as described by Althini et al., 2003, J Neurosci Res 72: 444-453). The coding sequence of Th is not affected, nor are the expression levels, so both the TH and cre recombinase proteins are produced in Th-expressing cells of this mouse line. |
| Phenotypic information | No phenotypic manifestations noted. |
| Breeding history | Generated by blastocyst injection of 129/SvJ ES cells into C57BL/6J blastocysts. The frt-flanked neo cassette has been eliminated by crossing with FLP-deleter mice (Susan Dymecki). Maintained since year 2000 by backcrossing to our colony of C57BL/6J originating from Bomholtgard (Denmark). Currently we maintain neo-minus N11 mice. |
| References |
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Information from EMMA
| Archiving centre | Karolinska Institutet, Stockholm, Sweden |
| Animals used for archiving | heterozygous , wild-type C57BL/6J |
| Stage of embryos | 2-cell |
Literature references
- Transgenic expression of Cre recombinase from the tyrosine hydroxylase locus.;Lindeberg Jonas, Usoskin Dmitry, Bengtsson Henrik, Gustafsson Anna, Kylberg Annika, Söderström Stine, Ebendal Ted, ;2004;Genesis (New York, N.Y. : 2000);40;67-73; 15452869
- Optogenetic interrogation of dopaminergic modulation of the multiple phases of reward-seeking behavior.;Adamantidis Antoine R, Tsai Hsing-Chen, Boutrel Benjamin, Zhang Feng, Stuber Garret D, Budygin Evgeny A, Touriño Clara, Bonci Antonello, Deisseroth Karl, de Lecea Luis, ;2011;The Journal of neuroscience : the official journal of the Society for Neuroscience;31;10829-35; 21795535
- Dopamine triggers heterosynaptic plasticity.;Ishikawa Masago, Otaka Mami, Huang Yanhua H, Neumann Peter A, Winters Bradley D, Grace Anthony A, Schlüter Oliver M, Dong Yan, ;2013;The Journal of neuroscience : the official journal of the Society for Neuroscience;33;6759-65; 23595734
- A locus coeruleus to dentate gyrus noradrenergic circuit modulates aversive contextual processing.;Seo Dong-Oh, Zhang Eric T, Piantadosi Sean C, Marcus David J, Motard Laura E, Kan Bryce K, Gomez Adrian M, Nguyen Tammy K, Xia Li, Bruchas Michael R, ;2021;Neuron;109;2116-2130.e6; 34081911