B6.Cg-Hprttm1(Cd68-BCL2)Ple/Orl
| Status | Available to order |
| EMMA ID | EM:04748 |
| International strain name | B6.Cg-Hprttm1(Cd68-BCL2)Ple/Orl |
| Alternative name | Tg CD68 hBcl2 |
| Strain type | Targeted Mutant Strains : Knock-in |
| Allele/Transgene symbol | Hprttm1(Cd68-BCL2)Ple, |
| Gene/Transgene symbol | Hprt1 |
Information from provider
| Provider | philippe Lesnik |
| Provider affiliation | INSERM Unit 939, Hôpital de la Pitié-Salpêtrière |
| Genetic information | Using homologous recombination in embryonic stem (ES) cells, a transgene made of the anti-apoptotic protein hBcl2 under the macrophage-specific promoter Cd68 was inserted at a defined site in the mouse genome as described previously (Farhadi et al). Site-specific and single-copy integration of the transgene was achieved at the hypoxanthine phosphoribosyl transferase (Hprt) locus on the X chromosome. |
| Phenotypic information | Macrophages with enhanced longevity and resistance to apoptosis. Immune system is functional. |
| Breeding history | The transgene was introduced by electroporation into BPES ES cells (hybrid C57BL/6 x 129). Targeted ES cells were injected into blastocysts, implanted into pseudopregnant females and chimeric mice generated. These chimeras transmitted the transgene through their germ line, and a number of mice bearing the transgene were obtained. Then, the transgenic mice were backcrossed 6 times on C57BL/6 genetic background. Currently continuing the backcross on C57BL/6. |
| References |
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| Homozygous fertile | not known |
| Homozygous viable | not known |
| Homozygous matings required | no |
| Immunocompromised | no |
Information from EMMA
| Archiving centre | Institut de Transgenose, INTRAGENE, Orléans, France |
| Animals used for archiving | heterozygous C57BL/6J |
Literature references
- Macrophage apoptosis exerts divergent effects on atherogenesis as a function of lesion stage.;Gautier Emmanuel L, Huby Thierry, Witztum Joseph L, Ouzilleau Betty, Miller Elizabeth R, Saint-Charles Flora, Aucouturier Pierre, Chapman M John, Lesnik Philippe, ;2009;Circulation;119;1795-804; 19307478
- A combinatorial network of evolutionarily conserved myelin basic protein regulatory sequences confers distinct glial-specific phenotypes.;Farhadi Hooman F, Lepage Pierre, Forghani Reza, Friedman Hana C H, Orfali Wayel, Jasmin Luc, Miller Webb, Hudson Thomas J, Peterson Alan C, ;2003;The Journal of neuroscience : the official journal of the Society for Neuroscience;23;10214-23; 14614079