B6Brd;B6N-Tyrc-Brd Myh9tm1a(EUCOMM)Wtsi/WtsiCnbc

Status

Available to order

EMMA IDEM:05292
International strain nameB6Brd;B6N-Tyrc-Brd Myh9tm1a(EUCOMM)Wtsi/WtsiCnbc
Alternative nameEPD0070_5_C01
Strain typeTargeted Mutant Strains
Allele/Transgene symbolMyh9tm1a(EUCOMM)Wtsi
Gene/Transgene symbolMyh9
DisclaimerPlease note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA:
  1. We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted.
  2. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure.
  3. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA.
  4. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider Wellcome Trust Sanger Institute
Provider affiliationWellcome Trust Sanger Institute
Genetic informationThis mouse line originates from EUCOMM ES clone EPD0070_5_C01. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic informationPotential phenotyping data in the IMPC portal
References
  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information from EMMA

Archiving centreCNB-CSIC, Centro Nacional de Biotecnologia, Madrid, Spain

Disease and phenotype information

Orphanet associated rare diseases, based on orthologous gene matching

IMPC phenotypes (allele matching)
  • increased bone mineral density / IMPC
  • increased circulating aspartate transaminase level / IMPC
  • preweaning lethality, complete penetrance / IMPC
IMPC phenotypes (gene matching)
  • increased circulating aspartate transaminase level / IMPC
  • increased bone mineral density / IMPC
  • preweaning lethality, complete penetrance / IMPC
MGI phenotypes (gene matching)
  • abnormal thrombopoiesis / MGI
  • abnormal megakaryocyte differentiation / MGI
  • abnormal cell morphology / MGI
  • cataract / MGI
  • increased incidence of corneal inflammation / MGI
  • abnormal germ layer development / MGI
  • failure to gastrulate / MGI
  • decreased embryo size / MGI
  • abnormal placenta morphology / MGI
  • abnormal placenta labyrinth morphology / MGI
  • pale yolk sac / MGI
  • no abnormal phenotype detected / MGI
  • abnormal megakaryocyte morphology / MGI
  • abnormal blood coagulation / MGI
  • abnormal lens fiber morphology / MGI
  • albuminuria / MGI
  • no phenotypic analysis / MGI
  • abnormal cell migration / MGI
  • thrombocytopenia / MGI
  • abnormal vascular branching morphogenesis / MGI
  • abnormal vitelline vasculature morphology / MGI
  • abnormal cell adhesion / MGI
  • embryonic growth retardation / MGI
  • abnormal spongiotrophoblast layer morphology / MGI
  • abnormal placenta size / MGI
  • abnormal platelet morphology / MGI
  • increased susceptibility to age-related hearing loss / MGI
  • abnormal brain vasculature morphology / MGI
  • abnormal neutrophil morphology / MGI
  • hematuria / MGI
  • abnormal eye physiology / MGI
  • glomerulosclerosis / MGI
  • renal/urinary system phenotype / MGI
  • homeostasis/metabolism phenotype / MGI
  • hearing/vestibular/ear phenotype / MGI
  • embryo phenotype / MGI
  • vision/eye phenotype / MGI
  • hematopoietic system phenotype / MGI
  • corneal vascularization / MGI
  • increased bleeding time / MGI
  • impaired hearing / MGI
  • podocyte foot process effacement / MGI
  • increased megakaryocyte cell number / MGI
  • abnormal placental labyrinth vasculature morphology / MGI
  • prenatal lethality, complete penetrance / MGI
  • embryonic lethality between implantation and somite formation, complete penetrance / MGI
  • embryonic lethality during organogenesis, complete penetrance / MGI
  • preweaning lethality, complete penetrance / MGI
  • abnormal visceral endoderm morphology / MGI
  • thin placenta labyrinth / MGI
  • abnormal placenta intervillous maternal lacunae morphology / MGI
  • abnormal placenta fetal blood space morphology / MGI
  • increased or absent threshold for auditory brainstem response / MGI

Literature references

  • Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes.;White Jacqueline K, Gerdin Anna-Karin, Karp Natasha A, Ryder Ed, Buljan Marija, Bussell James N, Salisbury Jennifer, Clare Simon, Ingham Neil J, Podrini Christine, Houghton Richard, Estabel Jeanne, Bottomley Joanna R, Melvin David G, Sunter David, Adams Niels C, null null, Tannahill David, Logan Darren W, Macarthur Daniel G, Flint Jonathan, Mahajan Vinit B, Tsang Stephen H, Smyth Ian, Watt Fiona M, Skarnes William C, Dougan Gordon, Adams David J, Ramirez-Solis Ramiro, Bradley Allan, Steel Karen P, ;2013;Cell;154;452-64; 23870131

Information on how we integrate external resources can be found here

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Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
Distribution of this strain is subject to a provider MTA. Both signing of the MTA and submission of the online EMMA Mutant Request Form are required before material can be shipped.

EMMA conditions
Legally binding conditions for the transfer

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