129S9.B6-a/H
| Status | Available to order |
| EMMA ID | EM:05339 |
| International strain name | 129S9.B6-a/H |
| Alternative name | 129S9/SvEvH.B6-a/H |
| Strain type | Spontaneous |
| Allele/Transgene symbol | a, |
| Gene/Transgene symbol | a |
Information from provider
| Provider | Sara Wells |
| Provider affiliation | MRC Mammalian Genetics Unit |
| Genetic information | The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a). When chimaeric mice derived from 129S9/SvEvH ES cells are tested for germ line transmission (GLT) it is often performed against a C57BL/6J background to use the non-agouti phenotype as a marker of non-GLT. Mice with Agouti coats are derived from the ES cells and therefore potential carriers of the genetic alteration. These mice however are F1s between C57BL/6 and 129S9/SvEvH. By transferring the a locus to the 129S9/SvEvH background we be able to maintain the 129 genetic background whilst checking for GLT. This strain allows chimaeric mice derived from 129S9/SvEvH ES cells to be tested for germline transmission using coat colour but ensuring that the genetic background is maintained as 129S9/SvEvH. |
| Phenotypic information | Black coat colour. The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a). |
| Breeding history | The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a) and maintained by brother x sister mating. |
| References |
|
| Homozygous fertile | yes |
| Homozygous viable | yes |
| Homozygous matings required | yes |
| Immunocompromised | not known |
Information from EMMA
| Archiving centre | Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom |
| Animals used for archiving | homozygous 0, homozygous 0 |
| Breeding at archiving centre | The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a). |
| Stage of embryos | 2-cell |
Disease and phenotype information
MGI allele-associated human disease models
Literature references
- A new coat color mouse line for testing germline transmission of embryonic stem cells while retaining an inbred genetic background.;Barbaric Ivana, Stewart Michelle, Wells Sara, Dear T Neil, ;2007;Journal of the American Association for Laboratory Animal Science : JAALAS;46;37-40; 17487951