B6Brd;B6N-Tyr^c-Brd Ppp5c^{tm1a(EUCOMM)Wtsi}/WtsiH

Status	Available to order
EMMA ID	EM:05851
International strain name	B6Brd;B6N-Tyr^c-Brd Ppp5c^{tm1a(EUCOMM)Wtsi}/WtsiH
Alternative name	EPD0114_3_G12
Strain type	Targeted Mutant Strains
Allele/Transgene symbol	Ppp5c^{tm1a(EUCOMM)Wtsi},
Gene/Transgene symbol	Ppp5c
Disclaimer	Please note that for EUCOMM and KOMP-CSD mice supplied to the scientific community by INFRAFRONTIER/EMMA: We can not guarantee a null mutation for Knock-out first alleles (tm1a alleles, see http://www.mousephenotype.org/about-ikmc/targeting-strategies) as the critical exon has not been deleted. That the structure of the targeted mutation in the ES cells obtained from EUCOMM/KOMP to generate EUCOMM/KOMP mice is not verified by INFRAFRONTIER/EMMA. It is recommended that the recipient confirms the mutation structure. No check for determining the copy number of the targeting construct in ES cells obtained from EUCOMM/KOMP is done by INFRAFRONTIER/EMMA. The level of quality control before mice are released is to confirm the individual mouse genotype by short range PCR.

Information from provider

Provider	Wellcome Trust Sanger Institute
Provider affiliation	Wellcome Trust Sanger Institute
Genetic information	This mouse line originates from EUCOMM ES clone EPD0114_3_G12. For further details on the construction of this clone see the page at the IMPC portal.
Phenotypic information	Potential phenotyping data in the IMPC portal
References	None available

Information from EMMA

Archiving centre	Mary Lyon Centre at MRC Harwell, Oxford, United Kingdom
Animals used for archiving	heterozygous C57BL/6Brd-Tyr^c-Brd or C57BL/6NTac
Breeding at archiving centre	Received as frozen sperm. May be carrying C57BL/6Brd-Tyr^c-Brd therefore carries the potential to produce albino offspring if intercrossed.

Disease and phenotype information

IMPC phenotypes (allele matching)

abnormal rib morphology / IMPC
decreased body length / IMPC
decreased body weight / IMPC
abnormal coat/hair pigmentation / IMPC
decreased circulating triglyceride level / IMPC
abnormal bone structure / IMPC
increased lean body mass / IMPC
vertebral fusion / IMPC
increased T cell number / IMPC
decreased circulating glucose level / IMPC
increased CD8-positive, alpha-beta T cell number / IMPC
increased total body fat amount / IMPC

MGI phenotypes (allele matching)

abnormal rib morphology / MGI
decreased circulating HDL cholesterol level / MGI
decreased body length / MGI
decreased body weight / MGI
abnormal coat/hair pigmentation / MGI
decreased circulating triglyceride level / MGI
decreased rib number / MGI
abnormal bone structure / MGI
decreased lumbar vertebrae number / MGI
abnormal behavior / MGI
decreased circulating cholesterol level / MGI
decreased circulating glucose level / MGI
decreased mean corpuscular hemoglobin concentration / MGI
lymphoid hyperplasia / MGI
vertebral transformation / MGI
vertebral fusion / MGI
caudal vertebral transformation / MGI
caudal vertebral fusion / MGI
cervical vertebral fusion / MGI
decreased thoracic vertebrae number / MGI
truncated ribs / MGI
increased T cell number / MGI
increased CD4-positive, alpha beta T cell number / MGI
increased CD8-positive, alpha-beta T cell number / MGI
decreased respiratory quotient / MGI
increased lymphoma incidence / MGI

Order

Availabilities

Requesting frozen sperm or embryos is generally advisable wherever possible, in order to minimise the shipment of live mice.

Frozen sperm. Delivered in 4 weeks (after paperwork in place). €1740^*
Rederivation of mice from frozen stock, delivery time available upon request . €3880^*
LacZ tagged null allele (tm1b allele), service fee and delivery time available upon request
Conditional ready allele (tm1c allele), service fee and delivery time available upon request
Reporterless null allele (tm1d allele), service fee and delivery time available upon request
Tissue - Types of tissue, service fee and delivery time available upon request

Due to the dynamic nature of our processes strain availability may change at short notice. The local repository manager will advise you in these circumstances.

^* In addition users have to cover all the shipping costs (including the cost for returning dry-shippers, where applicable).

More details on pricing and delivery times

Practical information

Genotyping protocol

Example health report
(Current health report will be provided later)

Material Transfer Agreement (MTA)
MTA will be issued after an order has been submitted.

EMMA conditions
Legally binding conditions for the transfer

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B6Brd;B6N-Tyrc-Brd Ppp5ctm1a(EUCOMM)Wtsi/WtsiH